FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120708
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT83260.39260473389075723No Hit
GTATAAGAATTTCCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAGGCGAACTGTGGCTGCACCAT78360.36949924270573786No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATCAGGCGT68610.32352403065391366No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65760.31008512251568815No Hit
TTCTTATACTGTTGGCAGTAATAAATTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGACTGAATTCTGTC63690.30032423134160857No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT59970.282782919666451No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC59020.2783032836203759No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55130.25996035286328906No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45910.2164843061845384No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC45280.21351360017503587No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44760.21106158886560525No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44490.20978842914724707No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG44160.20823234504703148No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT42760.20163077613702596No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42460.20021615422773903No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41490.19564221005437804No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG41110.193850355635948No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38110.179704136543079No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG38100.17965698247943612No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGGCAGA37610.17734643336093417No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGGCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA37180.1753188086242896No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG36320.17126355915100053No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT35210.166029458086639No Hit
GAATTCAGTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAATTTATTACTGCCAACAGTATAAGAATTTC35080.16541645525928134No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34190.16121974359506352No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33890.15980512168577665No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA32870.15499540719420118No Hit
CTAATACTCTGCCTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA32570.15358078528491428No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT32470.15310924464848533No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT32300.15230762556655608No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31570.14886537892062462No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCCTGATTTCCACCTTGGTCCCTTGGCCGAATGTCCACGGGAAATT31500.14853530047512434No Hit
GTGCAGCCACAGTTCGCCTGATTTCCACCTTGGTCCCTTGGCCGAATGTC31170.14697921637490877No Hit
CTACTAATACTCTGCCTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA30730.1449044375746213No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30420.1434426616016915No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30090.14188657750147593No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA29740.14023618527397452No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28790.13575654922789937No Hit
CAGTAATACATGGCGCTGTCCGAGGCCTTCAGGCTGCTCCAATGCAGGTAGGCGGTGTTGATGGACTTGTCGG28620.13495493014597013No Hit
GTATTACTGTGTCCGGACGAGAGATGACTACCCAAGGGGATGGCTCGACCCCTGGGGCCAGGGAACCCTGGTCAC28180.13288015134568265No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC27700.13061675629082364No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA26570.12528834709917633No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26530.12509973084460474No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25710.12123309762588722No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25060.11816808348909894No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25000.11788515910724155No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG24910.11746077253445547No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24240.11430145027038141No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23920.11279252023380872No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23680.11166082270637918No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23380.1102462007970923No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGGCAGAGTATTAGTAG23200.10939742765152015No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC22960.10826573012409063No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22540.10628525945108896No Hit
ACCTTGATGGGACCCCACTTTGTAAACTAGACGCCTGATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG22470.10595518100558869No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC21830.10293732093244332No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG21690.10227716404144277No Hit
CAGTATAAGAATTTCCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAGGCGAACTGTGGCTGCACCAT21610.10189993153229958No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21510.10142839089587062No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21290.1003910014957269No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21210.10001376898658373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACG405.5729786E-542.34422
GTGGTAT5850.039.3764881
GTATCAA52250.035.398941
CTAACGC751.9268336E-631.615513
TATCAAC58400.031.4391172
ATCAACG58800.031.2813823
CAACGCA59800.030.7575575
TCAACGC59700.030.6970484
AACGCAG59950.030.5675936
TCGACGC450.00505632730.1107224
ACGCAGA62850.029.3727387
CGCAGAG63500.029.1254148
AGAGTAC64650.028.70805411
AATGGTA9050.028.36167770
AGGTACG500.00845764227.1002882
ATCGACG500.00845958927.0990093
TGTTAGA24600.026.5318470
CGTCTAG36600.026.04802170
GATTCAC3700.025.6354081
CCTAACG801.0830227E-425.406522
Produced by FastQC (version 0.11.5)