FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101282
Sequences flagged as poor quality0
Sequence length21-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA39060.3546775485298043No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC35020.3179930299414682No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA32560.2956554270386695No Hit
GTATATTACTGTGCGACTGAAGCGAAGGGGTGGTTATCCCTAGGGGCCGATTCGTACTACTGGGGCCAGGGAACC30550.2774039710083339No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28770.26124099004614626No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26070.23672410881136713No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCCACTGCCCCAGGTCTGACAGTAATAGTCGC25980.23590687943687447No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25090.22782538895578064No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24950.22655414326212542No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT23600.21429570264473585No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGTTCAC21230.19277532911642978No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20660.18759954307797638No Hit
CATCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG20620.18723633002264634No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20290.18423982231617334No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19800.1797904623883801No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGAT19770.17951805259688255No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19420.1763399383627445No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18500.1679860380901531No Hit
GCTTAACAGTGATGGCAGCCACATCAAGGGGGACGGGATCCCTGATCGCT18380.16689639892416291No Hit
CCCAAAGGCCACTGCCCCAGGTCTGACAGTAATAGTCGCCCTCATCCTCA17900.1625378422602022No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17850.16208382594103962No Hit
CTGTTAAGCTTCATCAAGTATCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC17610.15990454760905926No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17570.1595413345537292No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAACTATGCCATGAGCTGGGTCCGACAGGCTCCAGGGAAGGGG17400.15799767906857642No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCT17080.15509197462593596No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16760.15218627018329547No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16510.1499161885874826No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16150.14664727108951203No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16100.14619325477034947No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15940.14474040254902923No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT15840.14383236991070408No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15500.14074505894039854No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15410.1399278295659059No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15340.1392922067190783No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC15310.13901979692758076No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15220.13820256755308813No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15160.13765774797009303No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA15060.13674971533176788No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14900.13529686311044764No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGTTCACCTGCACT14830.13466124026362003No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14670.13320838804229979No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14580.13239115866780715No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14450.13121071623798447No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG14350.1303026835996593No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC14280.1296670607528317No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGGCGACTATTACTGTCAGAC14240.12930384769750164No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT13840.12567171714420103No Hit
GGCACATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTG13500.12258440617389552No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13400.12167637353557037No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13230.12013271805041761No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13140.11931548867592497No Hit
GTCTGAGGATGAGGGCGACTATTACTGTCAGACCTGGGGCAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAG12980.11786263645460474No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12720.11550175159495933No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12540.11386729284597406No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCCACTGCCCCAGGTCTGACAGTAATAGTCGCC12430.11286845694381639No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT12380.1124144406246538No Hit
CCTGCACTCTGACCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG12350.11214203083315627No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12290.11159721125016117No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA12220.11096158840333356No Hit
ATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT12140.11023516229267344No Hit
CTATTACTGTCAGACCTGGGGCAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC12110.1099627525011759No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12110.1099627525011759No Hit
CAGTAATAGTCGCCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC12090.10978114597351087No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12050.10941793291818082No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11980.10878231007135321No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11850.1076018676415305No Hit
CCCTTGATGTGGCTGCCATCACTGTTAAGCTTCATCAAGTATCGAGGGCC11800.10714785132236793No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11580.10515017951805261No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11500.10442375340739248No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11390.10342491750523482No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11270.10233527833924463No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11050.10033760653492929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATTA150.002512951667.8832716
TAACACG200.00731532551.82782442
GGTATCA5350.043.789111
TCTTATA1400.038.79222
TCGGCTA350.001460335938.7904420
TGGTATC1850.038.530092
GTGGTAT1900.037.5263671
TCTGGAG12950.037.2539870
GACAGGT1750.036.8626331
AGGGGGG2002.401066E-1036.0029170
TGAGTAC400.002811898733.9431764
TTCGGGG15600.033.69503470
GTATCAA10900.033.3294451
CTCTTAT1650.032.9235651
CCGGGGT602.625778E-432.5361968
GTAGTAG450.003817790731.8889460
CGTATGC1750.031.54072641
ACAGGTA1650.030.8658431
TGTGTTT950.004885960430.31824370
AGGTACG450.00500551130.1717113
Produced by FastQC (version 0.11.5)