FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089855
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCGAGAACTTTTGGCCAGGGGA55540.5096090764367737No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38500.3532580022113033No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30120.27636703965206383No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30040.2756329970500663No Hit
GTATGGTAGCTCACCGAGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT24140.22149735515274968No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGAGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA23980.22002926994875463No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23370.2144321951085236No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22660.2079175670157957No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGATACCA22010.2019534708745659No Hit
CAGTAATATAAAGCCGTGTCGTCGGCTCGCAGGCTGTTCATTTGCAGATA21360.19598937473333608No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGAAGCAACTACGTAGCCTGGTACC19860.1822260759458827No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19730.18103325671763676No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGTGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGAAG19400.17800533098439703No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19170.17589495850365414No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19000.17433511797440943No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18310.16800400053218087No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18030.1654348514251896No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17850.1637832555706952No Hit
ATATTACTGTGCGCGAGGACTTTGGGATATTGTGGTGGTGAGTCCTCTGG17310.15882846800721195No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17310.15882846800721195No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16690.15313963784173124No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16650.1527726165407325No Hit
GTCCCAGACCCACTGCCACTGATCCTGTCTGGGATGCCAGTGGCCCTGCT15960.14644149909850393No Hit
GTCTTTGTCTCCAGGGGAAAGTGTCACCCTCTCCTGCAGGGCCAGTCAGA15900.14589096714700578No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC15850.14543219052075734No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15500.1422207541370182No Hit
GTGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGAAGCAACTACGTA15080.1383670304765313No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14420.1323111790100518No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGTGTCACCCTCTCCTGC14090.12928325327681206No Hit
TCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGATCAGTGGCAGTGGGTCTGGGACAGACTTCACT13970.12818218937381579No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13740.12607181689307292No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA13280.12185107193158724No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13070.11992421010134376No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12940.1187313908730978No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCC12770.1171715503438531No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12550.11515293318835992No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12440.11414362461061334No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11940.10955585834812888No Hit
GTGTTAGAAGCAACTACGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCAT11850.10873006042088167No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11830.10854654977038229No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11810.10836303911988292No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11760.10790426249363448No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11740.10772075184313509No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11630.10671144326538852No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT11620.10661968794013882No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGATCCTGTCTGGGATGCCA11280.10350000688164938No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11210.10285771960490157No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10980.10074734712415873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)