FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089855
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCGAGAACTTTTGGCCAGGGGA50850.4665758288946695No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39320.3607819388817779No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA29190.2678337944038427No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23760.21801065279326148No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23490.21553325901151987No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGAGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA23320.21397341848227516No Hit
GTATGGTAGCTCACCGAGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT21620.19837501318982798No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20810.19094283184460317No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20710.1900252785921063No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20300.18626331025686904No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19920.18277660789738084No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGATACCA19660.18039096944088895No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19150.1757114478531548No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGAAGCAACTACGTAGCCTGGTACC18940.1737845860229113No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGTGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGAAG18170.16671942597868522No Hit
CAGTAATATAAAGCCGTGTCGTCGGCTCGCAGGCTGTTCATTTGCAGATA17910.16433378752219333No Hit
ATATTACTGTGCGCGAGGACTTTGGGATATTGTGGTGGTGAGTCCTCTGG17540.16093884048795484No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16990.15589229759922194No Hit
GTCTTTGTCTCCAGGGGAAAGTGTCACCCTCTCCTGCAGGGCCAGTCAGA16790.15405719109422814No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16420.15066224405998965No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16340.14992820145799213No Hit
GTCCCAGACCCACTGCCACTGATCCTGTCTGGGATGCCAGTGGCCCTGCT15360.1409361795835226No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15200.13946809437952756No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGTGTCACCCTCTCCTGC14690.1347885727917934No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14690.1347885727917934No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14680.13469681746654372No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14640.13432979616554494No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC14630.13423804084029528No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14560.13359575356354744No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14210.1303843171798083No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14050.12891623197581328No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA13640.12515426364057602No Hit
GTGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGAAGCAACTACGTA13480.123686178436581No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCC12920.11854788022259842No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12780.11726330566910277No Hit
TCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGATCAGTGGCAGTGGGTCTGGGACAGACTTCACT12120.11120745420262329No Hit
GTGTTAGAAGCAACTACGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCAT12040.11047341160062578No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT11720.10753724119263572No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11720.10753724119263572No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11720.10753724119263572No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGATCCTGTCTGGGATGCCA11270.10340825155639971No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11230.10304123025540095No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11200.10276596427965187No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAGTTATAGCATGAATTGGGTCCGCCAGGCTCCGGGGAAGGGG10980.10074734712415873No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10960.10056383647365935No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10960.10056383647365935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)