FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842700
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCACCAGTATGCTAGTTCCCCGCTCACTTTCGGCGCAGGGA125470.680903022738373No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT79550.4317034785911977No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGTCGATGGTGAGAGTGAAGTCTGCCCCAGAGCCACTGCCA72140.3914907472730233No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61390.3331524393552939No Hit
GTGTATTACTGTCACCAGTATGCTAGTTCCCCGCTCACTTTCGGCGCAGGGACCAAGGTGGACCTCAGACGAA54320.29478482661312205No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48680.2641775655288435No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45920.24919954414717535No Hit
CTCCTGCAGGGCCAGTCAGACTCTTCCCGCCAACTACTTAGCCTGGTACC44060.23910566017257287No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42310.22960872632550064No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41200.22358495685678623No Hit
GTAATACACTGCAAAATCCTCAGGCTCCAGTCTGTCGATGGTGAGAGTGAAGTCTGCCCCAGAGCCACTGCCA39560.21468497313724427No Hit
GTCAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATACCA38720.2101264448906496No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTCTTCCCGC38480.20882400824876543No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37440.2031801161339339No Hit
GTATGCTAGTTCCCCGCTCACTTTCGGCGCAGGGACCAAGGTGGACCTCAGACGAACTGTGGCTGCACCAT36630.19878439246757473No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGC35960.19514842350898137No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35900.19482281434851034No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA35260.1913496499701525No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34710.18836489933250122No Hit
GACCAAGGTGGACCTCAGACGAACTGTGGCTGCACCATCTGTCTTCATCT34660.188093558365442No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT33810.1834807619254355No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32810.17805394258425136No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31620.17159602756824224No Hit
CTACTTAGCCTGGTACCAGCAGAAATCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAGCAGGGCCA31470.17078200466706464No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31310.16991371357247517No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29270.15884300211645955No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29260.1587887339230477No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28920.1569436153470451No Hit
ACCTTGGTCCCTGCGCCGAAAGTGAGCGGGGAACTAGCATACTGGTGACAGTAATACACTGCAAAATCCTCAGG28270.1534161827752754No Hit
CTAGCATACTGGTGACAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGTCGATGGTGAGAGTGAAGTCTGCC27840.15108265045856623No Hit
GTGCAGCCACAGTTCGTCTGAGGTCCACCTTGGTCCCTGCGCCGAAAGTG27100.14706680414608997No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAGGTCCACCTTGGTCC26210.1422369349324361No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24730.1342052423074836No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23990.13018939599500734No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23960.13002659141477182No Hit
GAGTGAAGTCTGCCCCAGAGCCACTGCCACTGAACCTGTCTGGGATGCCA23820.129266836707006No Hit
CATCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGGCAGACTTCA23190.12584794052206003No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23090.12530525858794161No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22900.12427416291311663No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22860.12405709013946925No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22180.12036685298746405No Hit
TCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGGCAGACTTCACT22090.11987843924675748No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21970.11922722092581539No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATACCACCGGAGAAATTGTCTTGACGCAGTCTC21310.11564552016063384No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21240.11526564280675096No Hit
GACTTCACTCTCACCATCGACAGACTGGAGCCTGAGGATTTTGCAGTGTATTACTGTCACCAGTATGCTAGTTCC21020.11407174255169046No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20970.11380040158463126No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20840.11309491507027732No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20690.11228089216909969No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAGCAGGGCCACTGGCATCCCA20680.11222662397568785No Hit
GTCAGACTCTTCCCGCCAACTACTTAGCCTGGTACCAGCAGAAATCTGGC20490.11119552830086286No Hit
CTCTTCCTCCTGCTCCTCTGGCTCCCAGATACCACCGGAGAAATTGTCTT19700.10690834102132739No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19390.10522602702556032No Hit
ACCCTCTCCTGCAGGGCCAGTCAGACTCTTCCCGCCAACTACTTAGCCTGGTACCAGCAGAAATCTGGCCAGGCT19360.1050632224453248No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19340.10495468605850111No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGATTTCTGCTGGTACCAGGCTAAGTAGTTGGCGGGAAGAGTCTGA19080.10354371302979323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15750.042.6148451
CGTATGC8350.042.58280641
TCGTATG8300.041.52989640
TATACAC9350.040.791483
ACGAGAC9300.040.66890322
ATGCCGT8800.040.5413344
TTATACA9500.040.1474082
ATCTCGT7750.039.8914137
CTTATAC9700.039.690081
AATCTCG7800.039.60101336
CACGAGA9550.039.24321421
GATATAC350.001437141238.9184043
GGCAATC8600.038.6230933
CGAGACA10050.037.97707723
CCCACGA9950.037.65843619
GTATGCC9750.037.5822342
CCGTCTT9150.036.8739647
AGGCAAT10050.036.77753432
ATCTCCG10500.036.64816710
TCTCCGA10650.036.13199211
Produced by FastQC (version 0.11.5)