FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842700
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCACCAGTATGCTAGTTCCCCGCTCACTTTCGGCGCAGGGA117380.6370000542681934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84850.4604656210994736No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81180.44054919411732785No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGTCGATGGTGAGAGTGAAGTCTGCCCCAGAGCCACTGCCA69250.3758072393770011No Hit
GTGTATTACTGTCACCAGTATGCTAGTTCCCCGCTCACTTTCGGCGCAGGGACCAAGGTGGACCTCAGACGAA52830.28669886579475773No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49180.2668909751994356No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48430.2628208606935475No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43630.23677212785586368No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43100.2338959136050361No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42330.22971726271232432No Hit
CTCCTGCAGGGCCAGTCAGACTCTTCCCGCCAACTACTTAGCCTGGTACC42220.22912031258479404No Hit
GTAATACACTGCAAAATCCTCAGGCTCCAGTCTGTCGATGGTGAGAGTGAAGTCTGCCCCAGAGCCACTGCCA38570.20931242198947198No Hit
GTCAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATACCA37600.20404840722852338No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTCTTCCCGC36770.19954414717534052No Hit
GTATGCTAGTTCCCCGCTCACTTTCGGCGCAGGGACCAAGGTGGACCTCAGACGAACTGTGGCTGCACCAT35970.1952026917023932No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA35530.1928148911922722No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGC34070.18489173495414338No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33950.18424051663320126No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32810.17805394258425136No Hit
GACCAAGGTGGACCTCAGACGAACTGTGGCTGCACCATCTGTCTTCATCT31120.1688826178976502No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28860.15661800618657407No Hit
ACCTTGGTCCCTGCGCCGAAAGTGAGCGGGGAACTAGCATACTGGTGACAGTAATACACTGCAAAATCCTCAGG28440.15433874206327672No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28400.15412166928962934No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28290.1535247191620991No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28270.1534161827752754No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28240.1532533781950399No Hit
GTGCAGCCACAGTTCGTCTGAGGTCCACCTTGGTCCCTGCGCCGAAAGTG27540.149454604656211No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27210.14766375427362025No Hit
CTACTTAGCCTGGTACCAGCAGAAATCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAGCAGGGCCA27000.14652412221197156No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26750.14516741737667554No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26630.14451619905573343No Hit
GAGTGAAGTCTGCCCCAGAGCCACTGCCACTGAACCTGTCTGGGATGCCA25920.1406631573234927No Hit
CTAGCATACTGGTGACAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGTCGATGGTGAGAGTGAAGTCTGCC25890.14050035274325717No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25260.13708145655831117No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAGGTCCACCTTGGTCC24630.13366256037336519No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23060.1251424540077061No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22590.12259184891734955No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATACCACCGGAGAAATTGTCTTGACGCAGTCTC22430.12172355782276009No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22250.12074673034134693No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21920.11895587995875619No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21580.11711076138275357No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21530.11683942041569437No Hit
TCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGGCAGACTTCACT21490.116622347642047No Hit
GACTTCACTCTCACCATCGACAGACTGGAGCCTGAGGATTTTGCAGTGTATTACTGTCACCAGTATGCTAGTTCC21320.11569978835404568No Hit
CTCTTCCTCCTGCTCCTCTGGCTCCCAGATACCACCGGAGAAATTGTCTT20920.11352906061757205No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20560.11157540565474575No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20380.11059857817333262No Hit
CATCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGGCAGACTTCA20330.11032723720627341No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20310.11021870081944973No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19980.10842785043685896No Hit
ACCCTCTCCTGCAGGGCCAGTCAGACTCTTCCCGCCAACTACTTAGCCTGGTACCAGCAGAAATCTGGCCAGGCT19510.10587724534650242No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG19380.10517175883214848No Hit
GTCAGACTCTTCCCGCCAACTACTTAGCCTGGTACCAGCAGAAATCTGGC19360.1050632224453248No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19200.10419493135073532No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAGCAGGGCCACTGGCATCCCA19150.10392359038367611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)