FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188514
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCATGTCTTCGG48080.40453877699379226No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT44440.3739122972047447No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA36430.30651721393269243No Hit
CAATAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATA35870.3018054478113005No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG35190.2960840175210389No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33940.2855666824286462No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32090.27000102649190505No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31000.26082991029133856No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC28930.2434132033783363No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC28870.24290837129390144No Hit
GTATTATTGTGTGAAAGATAAAAGTGGGGATCCCTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCAC25810.21716193498772418No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24900.20950531504046227No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24450.20571907440720094No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC24290.20437285551537465No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22670.19074238923563375No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22150.18636717783719836No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC21830.18367474005354587No Hit
CCGTAATAGGGATCCCCACTTTTATCTTTCACACAATAATACAAGGCGGT21670.1823285211617196No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGACCATTCAGGCTGTCATCCCATG20870.17559742670258827No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC20710.174251207810762No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGACCATTCAGG19770.16634217182128272No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT19630.16516423029093474No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19430.1634814566761519No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19410.16331317931467362No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG18140.15262756686080264No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18130.1525434281800635No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17330.1458123337209322No Hit
GTCTGAGGATGAGGCTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCATGTCTTCGGAACTGGGAC17290.14547577899797562No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGACCATTCAGGC17040.1433723119794971No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16820.14152126100323598No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16680.140343319472888No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16480.13866054585810517No Hit
CCCCTGAGGGCCGCTGATTATTATTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAAGTAA15960.1342853344596698No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT15760.13260256084488697No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT15740.1324342834834087No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15700.13209772876045212No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15700.13209772876045212No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15650.13167703535675643No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15530.1306673711878867No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15530.1306673711878867No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15300.12873218153088645No Hit
GTCTGAGAACTGAGGACACCGCCTTGTATTATTGTGTGAAAGATAAAAGT15230.12814321076571247No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT15150.12747010131979936No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15140.12738596263906019No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTTCAG14670.12343144464432056No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14230.11972934269179833No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC14210.11956106533032006No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT14120.11880381720366777No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14090.11855140116145035No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13810.11619551810075439No Hit
GGGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAACTGGTGGAAT13630.11468102184744983No Hit
CTCCAGAGCCTTCCCCGGAGCTTGACGGACCCAGTGCATGATATGATCAC13540.11392377372079757No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA13530.11383963504005842No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG13380.1125775548289713No Hit
GCACTGAACACAGAGAACGCACCATGGGGTTTGGACTGAGCTGGGTTTTC13220.11123133593714503No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13210.1111471972564059No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13030.10963270100310135No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC12880.10837062079201423No Hit
GATTATTATTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGATGCTGG12850.1081182047497968No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC12840.10803406606905766No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12750.1072768179424054No Hit
GGATGACAGCCTGAATGGTCATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC12680.1066878471772314No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12610.1060988764120574No Hit
ATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG12550.10559404432762257No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12430.10458438015875286No Hit
CTCATGTGCAGCCTCTGGATTCACCTTTGGTGATCATATCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGCT12170.1023967744595352No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC12100.1018078036943612No Hit
TTATTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGATGCTGGTACCA12070.10155538765214377No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12040.10130297160992634No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11980.10079813952549149No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC11960.1006298621640132No Hit
GTCTATGCAGCCTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAG11950.10054572348327406No Hit
CTCTTGTTCTGGAAGCACCCCCAACATCGAAACTAATGCTGTTACTTGGTACCAGCATCTCCCAGGAACGGCCCC11930.10037744612179579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)