FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188514
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCATGTCTTCGG45880.3860282672311811No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39270.33041259926260863No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT39170.3295712124552172No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC34030.2863239305552984No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33180.2791721426924714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32090.27000102649190505No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA32020.26941205572673105No Hit
CAATAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATA31770.2673085887082525No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG28140.23676624759994414No Hit
GTATTATTGTGTGAAAGATAAAAGTGGGGATCCCTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCAC26810.22557580306163832No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25770.2168253802647676No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22410.18855478353641605No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22310.18771339672902465No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21890.1841795721379807No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC21750.18300163060763272No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT20960.17635467482924055No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20810.17509259461815344No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20700.17416706913002286No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20680.17399879176854458No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG19840.1669311425864567No Hit
CCGTAATAGGGATCCCCACTTTTATCTTTCACACAATAATACAAGGCGGT19300.16238765382654308No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC19110.16078901889249939No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGACCATTCAGGCTGTCATCCCATG18820.15834899715106426No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18650.1569186395784989No Hit
GTCTGAGGATGAGGCTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCATGTCTTCGGAACTGGGAC18260.15363723102967236No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT17260.1452233629557582No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17060.14354058934097538No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16960.14269920253358395No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16650.14009090343067057No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16290.13706191092406148No Hit
GGGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAACTGGTGGAAT16140.13579983071297436No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT16130.1357156920322352No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG15990.1345377505018872No Hit
GTCTGAGAACTGAGGACACCGCCTTGTATTATTGTGTGAAAGATAAAAGT15920.13394877973671324No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15890.1336963636944958No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15650.13167703535675643No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC15640.13159289667601728No Hit
CCCCTGAGGGCCGCTGATTATTATTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAAGTAA15540.13075150986862585No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15400.12957356833827788No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15280.1285639041694082No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15110.12713354659684278No Hit
CTCTTGTTCTGGAAGCACCCCCAACATCGAAACTAATGCTGTTACTTGGTACCAGCATCTCCCAGGAACGGCCCC15030.12646043715092967No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14730.1239362767287554No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14690.12359972200579883No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGACCATTCAGG14630.12309488992136398No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT14510.1220852257524943No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG14340.12065486817992889No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTTCAG14180.11930864928810263No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14160.11914037192662433No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG14020.11796243039627635No Hit
CTCCAGAGCCTTCCCCGGAGCTTGACGGACCCAGTGCATGATATGATCAC14010.11787829171553722No Hit
ATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG13850.11653207282371096No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13710.11535413129336297No Hit
GGATGACAGCCTGAATGGTCATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC13660.11493343788966726No Hit
GATTATTATTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGATGCTGG13520.1137554963593193No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13400.11274583219044959No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC13190.11097891989492763No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGACCATTCAGGC12960.10904373023792736No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12790.10761337266536196No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12440.104668518839492No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12410.10441610279727456No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12300.10349057730914403No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12260.10315402258618746No Hit
GTTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAAGTAACAGCATTAGTT12170.1023967744595352No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12050.1013871102906655No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA12000.10096641688696976No Hit
GCACTGAACACAGAGAACGCACCATGGGGTTTGGACTGAGCTGGGTTTTC11940.10046158480253493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCAG502.208722E-456.90124570
GTGGTAT2200.052.913651
TGGTATC2650.045.216512
GGTATCA10150.043.177291
GTATCAA26500.040.0522841
ACAGGTT1050.039.129421
TATAAGA901.3278623E-1038.039292
GACAGGT2000.037.662071
TATCAAC29800.035.499082
AATGGTA3650.035.07610770
AAAGCGA601.4079509E-534.26132220
TGTAGCG400.002696273834.2353632
ATCAACG30850.034.179873
TCAACGC31200.033.90764
AACGCAG31650.033.639026
CAACGCA31650.033.4240955
ATAAGAG1056.9849193E-1032.6051063
GTATAAG959.475116E-932.436231
ACGCAGA33050.032.214077
CGCAGAG33550.031.6319398
Produced by FastQC (version 0.11.5)