FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491694
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCTTCAGACTGGAGTCCAGAGATGAGTAAAATGC61670.41342259203295045No Hit
GACTATTACTGTATGATTTGGCATACCAGCGCTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAG44820.30046376803821695No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43980.2948325863079157No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC43100.2889332530666477No Hit
GTAATACACAGCCGTGTCGTCGTCTCTCAGGCGATTCATTTGTAGATACA42820.2870561924898806No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42150.28256465468118797No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC40840.27378269269702765No Hit
GTGTTGACTCAGCCGTCTTCCCTCTCTGCCTCTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGGC40000.2681515109667264No Hit
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCACTATCTCCTG37560.2517942687977561No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36650.24569382192326308No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA34240.2295376933875178No Hit
TCTCTGGACTCCAGTCTGAAGATGAGGCTGACTATTACTGTATGATTTGG34190.2292025039988094No Hit
CACCTGCACCTTACGCAGTGGCCTCAATGTTGGCAGCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC31200.2091581785540466No Hit
CAGTAATAGTCAGCCTCATCTTCAGACTGGAGTCCAGAGATGAGTAAAAT31160.20889002704307988No Hit
GGTTCCCTCTCGCAGGCTGTGTTGACTCAGCCGTCTTCCCTCTCTGCCTCTCCTGGAGCATCAGCCAGTCTCAC29940.2007114059585947No Hit
CAGATAACCAGCGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA29500.1977617393379607No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29170.19554948937248523No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29020.19454392120636002No Hit
GTGTATTACTGTGTGAGAGATGGCCTTTATTTTGACTTTTGGGGCCAGGG28900.19373946667345984No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAGCGCTGGTATGCCAAATCATACAGTAATAGTCAG28590.1916612924634677No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27460.18408601227865767No Hit
CAGTAATACACAGCCGTGTCGTCGTCTCTCAGGCGATTCATTTGTAGATA27230.18254414109059902No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT27030.18120338353576537No Hit
GTACAAATCAGACTCAGATAACCAGCGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGGTTCGGC26990.18093523202479866No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC26830.17986262598093175No Hit
CTCCAGAGCCCCGCTGGTTATCTGAGTCTGATTTGTACCGCAGGAGATAGTGGGGAGGACTCCCTGGCTTCTG26540.17791852752642298No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26440.17724814874900616No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26070.17476774727256394No Hit
CTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGGCCTCAAT26010.17436552000611386No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGGTTCGGCCAATGCAGGCATTTTACTCATCTCTGGA26010.17436552000611386No Hit
CTATTACTGTATGATTTGGCATACCAGCGCTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCC25630.17181808065192997No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25470.17074547460806305No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25260.16933767917548773No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT25110.16833211100936252No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24560.16464502773357004No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23980.16075683082455247No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23340.15646640664908487No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23050.1545223081945761No Hit
GAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA22610.1515726415739421No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22430.15036595977459183No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22220.14895816434201653No Hit
GATTTGTACCGCAGGAGATAGTGGGGAGGACTCCCTGGCTTCTGCTGGTA22200.14882408858653318No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22080.148019634053633No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC21860.146544800743316No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21640.145069967432999No Hit
GGTTATCTGAGTCTGATTTGTACCGCAGGAGATAGTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATC21540.14439958865558217No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC20910.14017620235785622No Hit
GTCCAGAGATGAGTAAAATGCCTGCATTGGCCGAACCATCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGC20870.1399080508468895No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAG20600.13809802814786412No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20550.1377628387591557No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGTTGACTCAGCCGTCTTCCCT20280.1359528160601303No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGAGGCAGAGAGGGAAGACGG19930.13360649033917144No Hit
CTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCACTATC19810.13280203580627126No Hit
CCACTATCTCCTGCGGTACAAATCAGACTCAGATAACCAGCGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATC19320.12951717979692887No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19190.12864568738628698No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19140.12831049799757857No Hit
GCTTGGTCCCTCCGCCGAAAACCACAGCGCTGGTATGCCAAATCATACAGTAATAGTCAGCCTCATCTTCAGA18830.12623232378758648No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18790.12596417227661974No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18580.12455637684404441No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18450.12368488443340257No Hit
GTATTACTGTGTGAGAGATGGCCTTTATTTTGACTTTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCTGCATC17950.12033299054631849No Hit
CCTTACGCAGTGGCCTCAATGTTGGCAGCTACAGGATATACTGGTACCAG17900.11999780115761008No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGTTGACTCAGCCGTCTTCCCTCTCTGCCTCTCCTGGAGCATCAGCCAGT17420.11677998302600935No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGTTGACTCAGCCGTCTTCCCTCTCTGCCTCTCCTGGAGCATCAG17310.11604256637085086No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16990.11389735428311704No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16670.11175214219538324No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT16590.11121583917344978No Hit
CTCGCAGGCTGTGTTGACTCAGCCGTCTTCCCTCTCTGCCTCTCCTGGAG16550.11094768766248306No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16310.10933877859668269No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16300.10927174071894102No Hit
ATATTACGCAGACTCTGTGCAGGGCCGATTCACCATCTCCAGGGACAACG16120.10806505891959074No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15850.10625503622056535No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15560.10431093776605657No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15510.10397574837734816No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGTTGACTCAGCCGTCTTCCCTCT15500.1039087104996065No Hit
GTATATCCTGTAGCTGCCAACATTGAGGCCACTGCGTAAGGTGCAGGTGA15460.10364055898863976No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC15450.10357352111089808No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15440.1035064832331564No Hit
GTCTCACCTGCACCTTACGCAGTGGCCTCAATGTTGGCAGCTACAGGATA15330.1027690665779979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4700.063.72827570
GTCTTAA200.00765711751.23361
ATCTCGT5300.045.36540637
TATCTCG5250.045.1097436
ATGCCGT6000.044.37787244
TCTCGTA5350.044.33080338
CGTATGC5850.044.18502441
TCGTATG5850.044.1491740
GCCTATC5300.043.96102533
GGTATCA12000.042.6946681
CTTATAC6500.042.56331
ACGAGAC6450.042.3509222
TTATACA6750.040.969022
CACGAGA6550.040.6617321
GACCGGA6650.040.59095826
CCCACGA6650.040.56102419
CGAGACC6750.040.4727323
GTGGTAT3650.040.2382621
TCTCCGA6500.039.9212111
TATACAC6950.039.7900543
Produced by FastQC (version 0.11.5)