FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491694
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59570.3993446377071973No Hit
GTAATAGTCAGCCTCATCTTCAGACTGGAGTCCAGAGATGAGTAAAATGC58220.3902945242120703No Hit
GACTATTACTGTATGATTTGGCATACCAGCGCTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAG44150.2959722302295243No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43910.29436332116372393No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39780.26667667765640946No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC39480.264665541324159No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC39010.2615147610702999No Hit
GTAATACACAGCCGTGTCGTCGTCTCTCAGGCGATTCATTTGTAGATACA37980.2546098596629067No Hit
GTGTTGACTCAGCCGTCTTCCCTCTCTGCCTCTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGGC35540.2382526174939364No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA34040.22819693583268416No Hit
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCACTATCTCCTG33250.22290094349109135No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32300.21653234510563157No Hit
TCTCTGGACTCCAGTCTGAAGATGAGGCTGACTATTACTGTATGATTTGG31520.21130339064178044No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30750.20614147405567093No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29050.19474503483958508No Hit
GGTTCCCTCTCGCAGGCTGTGTTGACTCAGCCGTCTTCCCTCTCTGCCTCTCCTGGAGCATCAGCCAGTCTCAC28730.19259982275185125No Hit
CAGTAATAGTCAGCCTCATCTTCAGACTGGAGTCCAGAGATGAGTAAAAT28730.19259982275185125No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAGCGCTGGTATGCCAAATCATACAGTAATAGTCAG28700.19239870911862622No Hit
CACCTGCACCTTACGCAGTGGCCTCAATGTTGGCAGCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC28530.19125906519701763No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC27600.18502454256704123No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27440.18395193652317432No Hit
GTGTATTACTGTGTGAGAGATGGCCTTTATTTTGACTTTTGGGGCCAGGG27410.18375082288994926No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26900.18033189112512352No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT26460.1773822245044895No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26130.17516997453901403No Hit
CTCCAGAGCCCCGCTGGTTATCTGAGTCTGATTTGTACCGCAGGAGATAGTGGGGAGGACTCCCTGGCTTCTG26080.17483478515030565No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25990.17423144425063047No Hit
GTACAAATCAGACTCAGATAACCAGCGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGGTTCGGC25430.17047732309709632No Hit
CAGATAACCAGCGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA25240.16920360342000437No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24710.16565059589969525No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGGTTCGGCCAATGCAGGCATTTTACTCATCTCTGGA24220.16236573989035283No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24070.16136017172422762No Hit
CTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGGCCTCAAT24000.16089090658003585No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23920.1603546035581024No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23830.15975126265842726No Hit
CTATTACTGTATGATTTGGCATACCAGCGCTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCC23700.1588797702477854No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC23330.1563993687713432No Hit
CAGTAATACACAGCCGTGTCGTCGTCTCTCAGGCGATTCATTTGTAGATA23270.1559971415048931No Hit
GGTTATCTGAGTCTGATTTGTACCGCAGGAGATAGTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATC23080.15472342182780116No Hit
GAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA22680.1520419067181339No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22070.1479525961758913No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22050.14781852042040794No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21680.14533811894396573No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC20890.14004212660237286No Hit
GATTTGTACCGCAGGAGATAGTGGGGAGGACTCCCTGGCTTCTGCTGGTA20670.1385672932920559No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20650.13843321753657253No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAG20260.13581874030464694No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGTTGACTCAGCCGTCTTCCCT19900.13340537670594638No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19750.13239980853982117No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19640.13166239188466267No Hit
GTATATCCTGTAGCTGCCAACATTGAGGCCACTGCGTAAGGTGCAGGTGA19430.13025459645208737No Hit
GTCCAGAGATGAGTAAAATGCCTGCATTGGCCGAACCATCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGC19320.12951717979692887No Hit
GCTTGGTCCCTCCGCCGAAAACCACAGCGCTGGTATGCCAAATCATACAGTAATAGTCAGCCTCATCTTCAGA18830.12623232378758648No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18580.12455637684404441No Hit
CCACTATCTCCTGCGGTACAAATCAGACTCAGATAACCAGCGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATC18400.12334969504469416No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGAGGCAGAGAGGGAAGACGG18370.1231485814114691No Hit
CTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCACTATC18110.12140559659018539No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT18040.12093633144599361No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17540.11758443755890953No Hit
GTATTACTGTGTGAGAGATGGCCTTTATTTTGACTTTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCTGCATC17160.11503699820472563No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17050.11429958154956714No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16930.11349512701666696No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16740.112221407339575No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16690.11188621795086659No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16320.10940581647442438No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16000.10726060438669056No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15980.1071265286312072No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGTTGACTCAGCCGTCTTCCCTCTCTGCCTCTCCTGGAGCATCAG15890.10652318773153206No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15590.10451205139928163No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15460.10364055898863976No Hit
CCTTACGCAGTGGCCTCAATGTTGGCAGCTACAGGATATACTGGTACCAG15300.10256795294477286No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGTTGACTCAGCCGTCTTCCCTCTCTGCCTCTCCTGGAGCATCAGCCAGT15290.10250091506703116No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15050.10089200600123083No Hit
CTCGCAGGCTGTGTTGACTCAGCCGTCTTCCCTCTCTGCCTCTCCTGGAG15020.10069089236800577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACT200.00770407951.15441519
GGTATCA12350.046.3645971
GTGGTAT2750.044.61841
TGGTATC2950.040.4326252
GTATCAA30900.038.2749981
GGACGTA400.002756364934.0823554
TATCAAC34350.033.731692
GACAGGT3150.033.5424921
ATCAACG35750.032.6035653
TCAACGC35950.032.5180784
TTTGGAT28550.032.02853870
CAACGCA36650.031.8969945
AACGCAG36950.031.545786
ACAGGCC3000.030.6751481
AGGTACG450.004909309630.2923772
CGCAGAG38400.030.1760758
TCTGCAT14750.029.98080870
GCGATTC13400.029.86464931
GGCGATT13400.029.85158530
AGAGTAC39300.029.39435611
Produced by FastQC (version 0.11.5)