FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160482
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGGTCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAGGAACTGTGG63310.5455491769799101No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46720.40259133704788186No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA39270.3383938742694846No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35140.3028052136956885No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31280.26954317257829075No Hit
CATTTATGCTGCATCCACCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA29640.25541111365794555No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28530.2458461225594193No Hit
GTGTATTACTGTCAGCAGTATGGTAGGTCACTCACTTTCGGCGGAGGGAC27580.23765986891653637No Hit
GTAATACACGGCCGTATCTTCAGACTTCAGGCTGCTCAGCTCCATGTAGA27450.2365396447338261No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACGACTTAGCCTGGTACC26060.2245618630879238No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC25290.2179266890826398No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25000.2154277274442861No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT24580.21180854162322205No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24550.21155002835028894No Hit
GTATGGTAGGTCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTT24350.20982660653073465No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG24160.20818935580215808No Hit
ATGGTAGGTCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAGGA22720.1957807187013672No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGGT21330.18380293705546488No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21250.18311356832764317No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21000.18095929105320033No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT20790.1791496981426683No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20300.1749273146847603No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19810.1707049312268523No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC19580.16872299613436484No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19440.16751660086067685No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCACCTA19030.16398358613059055No Hit
ACCTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT18740.16148462449223686No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18660.16079525576441514No Hit
GTGTATTACTGTGCGGGGGCCTGGGCAACTTCCTACCATGAGTACTGGGGCCAGGGAACCCTGGTCACCGTCTC18550.15984737376366026No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18460.15907183394486085No Hit
GTCTACATGGAGCTGAGCAGCCTGAAGTCTGAAGATACGGCCGTGTATTACTGTGCGGGGGCCTGGGCAACTTC18270.15743458321628429No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17790.153298370849354No Hit
CAGTAATACACGGCCGTATCTTCAGACTTCAGGCTGCTCAGCTCCATGTA17230.14847278975460196No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16650.14347486647789454No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16510.14226847120420652No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16340.1408035626575854No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCC16160.13925248301998652No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16100.13873545647412022No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15830.13640883701772194No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15730.1355471261079448No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15480.13339284883350194No Hit
GTGTTAGCAGCAACGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCATTTATGCTGCATCCA15370.13244496683274706No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15320.1320141113778585No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14860.12805024119288366No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGGTCA14710.12675767482821793No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14580.12563745064550763No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG14570.1255512795545299No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC14540.1252927662815968No Hit
GCATAAATGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAA14460.12460339755377507No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14420.1242587131898642No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14390.12400019991693108No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14350.1236555155530202No Hit
CCATGTAGACTGTGCTCGTGGATCCGTCCGCGGTAATCGTGACTCTGCCCTGGAACTTCTGTGCGTAGTTTG14260.12287997573422078No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14050.12107038282368877No Hit
GTCAGAGTGTTAGCAGCAACGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCATTTATGCTG13890.11969164536804536No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC13840.11926078991315678No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC13660.11770971027555792No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA13630.11745119700262477No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13590.1171065126387139No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13190.11365966899960532No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13170.1134873268176499No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13130.11314264245373905No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13110.11297030027178363No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13010.11210858936200649No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12820.1104713386334299No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12730.10969579881463047No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12600.10857557463192019No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12370.10659363953943275No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12190.10504255990183388No Hit
GTATTACTGTGCGGGGGCCTGGGCAACTTCCTACCATGAGTACTGGGGCC12150.10469787553792304No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11980.1032329669913019No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11860.10219891389956932No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11790.1015957162627253No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC11780.10150954517174761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7800.042.1177521
GTATCAA19500.040.467781
GTCGTAT602.9630974E-740.15831439
GCCTTAC350.001476062538.706151
CTTATAC5350.037.982671
TTATACA5500.036.9276732
TATACAC5850.036.4542433
TCTTATA2100.035.462292
ACGAGAC5750.035.3358322
TCGTATG4400.035.27791640
CGAGACA5800.034.4547823
TGTCGTA807.347444E-834.36269438
ATCTCGT3600.034.287737
CGTATGC4500.033.7778641
ATGCCGT4600.033.1939144
CACGAGA6200.032.7697221
ATCAACG24600.032.3367353
CTCTTAT2200.032.3284341
ATGTCGT851.2757118E-732.27077537
TCTCCGA6350.031.984611
Produced by FastQC (version 0.11.5)