FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160482
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59530.512976504590334No Hit
GTATTACTGTCAGCAGTATGGTAGGTCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAGGAACTGTGG58090.5005678674895432No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47080.40569349632307955No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA35380.30487331987915367No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30070.2591164705699873No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29340.25282598092861414No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27540.23731518455262554No Hit
CATTTATGCTGCATCCACCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA27440.23645347364284838No Hit
GTGTATTACTGTCAGCAGTATGGTAGGTCACTCACTTTCGGCGGAGGGAC26880.23162789254809638No Hit
GTAATACACGGCCGTATCTTCAGACTTCAGGCTGCTCAGCTCCATGTAGA24330.2096542643487792No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT24330.2096542643487792No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACGACTTAGCCTGGTACC24060.20732764489238092No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23700.2042254856171832No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG23250.20034778652318605No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC23110.19914139124949806No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22980.19802116706678774No Hit
GTATGGTAGGTCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTT22900.19733179833896605No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21190.18259654178177687No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA20510.1767369075952923No Hit
ATGGTAGGTCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAGGA20380.175616683412582No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20110.1732900639561837No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC19810.1707049312268523No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19350.16674106104187744No Hit
GTGTATTACTGTGCGGGGGCCTGGGCAACTTCCTACCATGAGTACTGGGGCCAGGGAACCCTGGTCACCGTCTC19090.16450061267645685No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCACCTA19090.16450061267645685No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18360.1582101230350837No Hit
GTCTACATGGAGCTGAGCAGCCTGAAGTCTGAAGATACGGCCGTGTATTACTGTGCGGGGGCCTGGGCAACTTC17540.15114409357491113No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17470.15054089593806713No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16820.1449397750245157No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGGT16460.14183761574931797No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT16310.14054504938465223No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16270.14020036502074137No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16140.1390801408380311No Hit
ACCTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT16100.13873545647412022No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16060.1383907721102094No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15980.13770140338238765No Hit
CAGTAATACACGGCCGTATCTTCAGACTTCAGGCTGCTCAGCTCCATGTA15980.13770140338238765No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15640.13477158628914537No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCC15600.13442690192523452No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15290.13175559810492538No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGGTCA15010.12934280755754937No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14960.1289119521026608No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14680.1264991615552848No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14640.12615447719137393No Hit
GCATAAATGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAA14630.1260683061003962No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14520.12512042409964136No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14460.12460339755377507No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14380.12391402882595336No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC14340.12356934446204251No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14240.12270763355226535No Hit
GTGTTAGCAGCAACGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCATTTATGCTGCATCCA14060.1211565539146665No Hit
GTCAGAGTGTTAGCAGCAACGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCATTTATGCTG14030.12089804064173335No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13690.11796822354849104No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC13660.11770971027555792No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA13650.1176235391845802No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG13320.11477989318231561No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13310.11469372209133792No Hit
CCATGTAGACTGTGCTCGTGGATCCGTCCGCGGTAATCGTGACTCTGCCCTGGAACTTCTGTGCGTAGTTTG13190.11365966899960532No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13160.1134011557266722No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13030.1122809315439619No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12880.1109883651792962No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12870.11090219408831847No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC12710.10952345663267506No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12430.10711066608529904No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12120.10443936226498989No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11990.10331913808227959No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC11940.10288828262739103No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11860.10219891389956932No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11830.10194040062663617No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11820.10185422953565845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18900.039.7266431
GGTATCA7600.039.606561
TCAACGC22850.032.5660174
ATCAACG22950.032.4227183
AACGCAG22850.032.416596
CAACGCA22950.032.275345
AGAGTAC24400.031.04374111
ACGCAGA24000.031.0042257
CGCGGTA13250.030.27311170
CGCAGAG24550.030.1718588
GACAGCC2100.028.9897161
GGGACAT1302.4920155E-1028.6192865
ATTCACT1800.028.1844432
GGACATA1452.910383E-1127.9912766
GGGTTAT500.00852038627.0582337
GATTCAC1900.026.7010521
ACAGGTA1201.0324948E-725.3661
GACATAA1351.1641532E-825.053927
TATCAAC31100.024.903812
CAGAGTA30400.024.69847310
Produced by FastQC (version 0.11.5)