FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776248
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAATTCTGTC40940.5274087662705733No Hit
CTATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGG40460.5212251754593893No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34160.4400655460625985No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33100.4264101163545671No Hit
GAGTATCAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGTGT29740.38312498067627876No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTGATACTCTGA27560.35504117240881783No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25080.3230926198843668No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23390.30132122723665633No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23050.29694118374540096No Hit
TCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT22840.29423586276550795No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22220.2862487246343952No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21890.28199750595170614No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21150.2724644701177974No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20070.2585513907926333No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19920.2566190186641383No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19760.2545578217270769No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19540.25172367593861755No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19140.24657068359596415No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18900.24347888819037214No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATCAGTAGCTGGTTGGCCTGGTATCAGCAGAAA18700.24090239201904548No Hit
GAATTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA18640.24012944316764745No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA18350.23639352371922373No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18290.23562057486782575No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17160.22106337149982994No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT16770.2160392039657429No Hit
GGCCAAAAGAGTACCCCGGAGAATAACTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG16740.2156527295400439No Hit
ACTTATTACTGCCTACACTATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAA15210.19594253382939475No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15080.1942678113180324No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT14850.1913048407210067No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14790.1905318918696087No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14710.18950129340107802No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14620.188341870123981No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14520.18705362203831766No Hit
CTGATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA14430.18589419876122065No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTGCCACC14230.18331770258989397No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14210.1830600529727613No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14100.18164298007853158No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13920.1793241335243376No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13720.1767476373530109No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13300.17133699539322483No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13180.16979109769042883No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13100.16876049922189815No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTG12740.1641228061135101No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12510.16115983551648444No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12380.15948511300512205No Hit
ACCCAGTCTCCTGCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT11660.150209726788346No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11470.14776205542558563No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11300.14557203367995794No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11250.1449279096371263No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11090.14286671270006493No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11040.14222258865723325No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10940.1409343405715699No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCAT10600.13655429708031452No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10580.13629664746318187No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10300.1326895528233245No Hit
CCCCTAAACTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCA10290.13256072801475816No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10230.13178777916336015No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10230.13178777916336015No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGAG10220.13165895435479383No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10120.1303707062691305No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10030.12921128299203347No Hit
CACTATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA9800.12624831239500778No Hit
GTCTCCTGCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATCAGTAG9740.12547536354360977No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9730.12534653873504342No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9690.1248312395007781No Hit
ATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9370.1207088456266554No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9370.1207088456266554No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9320.12006472158382372No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACTCCA9290.11967824715812474No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9250.1191629479238594No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9230.11890529830672672No Hit
ACCTTGATGGGACTCCACTTTCTAAATTAGACACCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG9220.11877647349816038No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9180.11826117426389504No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9150.11787469983819605No Hit
ATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC9120.11748822541249704No Hit
CTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCATCAAGGTT9110.11735940060393071No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9060.11671527656109902No Hit
TAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9060.11671527656109902No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9060.11671527656109902No Hit
GTCTCCTACAGATGCAGACAGGGTGGCAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG9040.11645762694396636No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8960.11542702847543568No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG8870.11426760519833867No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8840.11388113077263967No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8830.11375230596407335No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC8820.113623481155507No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8630.11117580979274665No Hit
GTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG8550.11014521132421598No Hit
GGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTGCCACC8510.10962991208995064No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATTTTG8460.10898578804711895No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8430.10859931362141996No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8380.1079551895785883No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8380.1079551895785883No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC8340.10743989034432296No Hit
GTGGAGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT8140.10486339417299627No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7950.10241572281023589No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7920.1020292483845369No Hit
CCTGCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG7850.10112747472457255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA150.001678992175.1449966
CTCGGTT200.00570908455.17839463
CGGCTTG200.00816552250.4048425
GTCGTAT306.46064E-445.78042239
CTTATAC3900.041.3764761
CGGTCAT450.001115260140.97328669
TATACAC3900.040.4961973
CGTATGC3750.040.4812841
GCCGTCT3650.040.13521646
TCGTATG3800.039.85940
CTCTTAT858.731149E-1139.551041
TTATACA4000.039.483792
ATACACA4100.038.520774
ATGCCGT3700.038.49828744
CCGTCTT3950.038.06926747
TACACAT4250.037.1612175
ATCTCGT3500.037.1098137
AGGCAAT4050.036.8298632
CTTGAAA4200.036.8049757
GTATGCC4050.036.72064642
Produced by FastQC (version 0.11.5)