FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776248
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40270.5187775040966289No Hit
CTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAATTCTGTC39400.5075697457513578No Hit
CTATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGG37900.48824602446640764No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35650.4592604425389824No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTGATACTCTGA27230.35078995372612876No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26940.34705403427770504No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26840.34576578619204174No Hit
GAGTATCAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGTGT25800.3323680061011429No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23480.30248065051375334No Hit
TCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT21220.27336624377776175No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20940.2697591491379044No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20820.2682132514351084No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20780.267697952200843No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20430.26318908390102136No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19190.24721480763879583No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19170.24695715802166318No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18370.23665117333635644No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17570.22634518865104966No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17180.2213210211169626No Hit
GAATTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA16950.21835805051993695No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA16630.21423565664581423No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATCAGTAGCTGGTTGGCCTGGTATCAGCAGAAA16510.21268975894301823No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16210.20882501468602815No Hit
GGCCAAAAGAGTACCCCGGAGAATAACTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG16000.20611969370613517No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG15740.20277024868341045No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15450.19903432923498676No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15400.19839020519215508No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15320.1973596067236244No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15190.19568488421226204No Hit
ACTTATTACTGCCTACACTATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAA14810.19078954148674135No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14790.1905318918696087No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACTCCA14440.18602302356978698No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14310.18434830105842462No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14210.1830600529727613No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT13840.17829353505580692No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13810.1779070606301079No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13670.1761035133101792No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13610.1753305644587812No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTGCCACC13140.1692757984561635No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12740.1641228061135101No Hit
CTGATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA12700.16360750687924477No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTG12470.16064453628221909No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12400.15974276262225473No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12220.15742391606806072No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12130.15626449279096372No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11640.14995207717121334No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11530.14853500427698363No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11470.14776205542558563No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11440.14737558099988662No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11250.1449279096371263No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11090.14286671270006493No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11000.14170728942296792No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10720.13810019478311056No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10710.13797136997454423No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGAG10700.13784254516597788No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10220.13165895435479383No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10030.12921128299203347No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9900.12753656048067114No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9890.12740773567210478No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9890.12740773567210478No Hit
ATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC9700.12496006430934443No Hit
ACCTTGATGGGACTCCACTTTCTAAATTAGACACCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG9700.12496006430934443No Hit
ACCCAGTCTCCTGCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT9670.12457358988364542No Hit
CTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCATCAAGGTT9660.1244447650750791No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9560.12315651698941575No Hit
ATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9240.11903412311529304No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCAT9100.11723057579536436No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9070.11684410136966537No Hit
TAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT8930.11504055404973669No Hit
GTCTCCTACAGATGCAGACAGGGTGGCAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG8860.11413878038977233No Hit
CCCCTAAACTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCA8850.11400995558120601No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8690.11194875864414466No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG8620.11104698498418031No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8460.10898578804711895No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8450.10885696323855262No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT8380.1079551895785883No Hit
CACTATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA8370.10782636477002196No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8370.10782636477002196No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8360.10769753996145563No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8340.10743989034432296No Hit
GTCTCCTGCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATCAGTAG8320.10718224072719029No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8150.1049922189815626No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8130.10473456936442992No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8010.10318867166163391No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7950.10241572281023589No Hit
GTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG7940.10228689800166957No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7810.10061217549030721No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCG451.769118E-551.41266369
TACTTAC200.00817084150.3965387
GTGGTAT2350.040.0390361
GTACTAG350.001533543938.404791
GTCTATA451.1703244E-437.330774
GTATCAA15250.034.595791
ATCAACG17150.030.365263
TCAACGC17200.030.2769894
AACGCAG17500.029.9499426
TCGCTAT450.00522657429.90509824
TTGTATC450.00523647929.89352217
GATTCAC1800.029.8703941
CAACGCA17600.029.5888795
GCAGAGT18200.028.7980239
CGCAGAG18150.028.6922448
TATCAAC18200.028.6152652
ACGCAGA18550.028.2546637
GGTTTTC606.2867114E-428.03060219
TATAAGA606.3271576E-427.9998822
CAGTTCG16300.027.592470
Produced by FastQC (version 0.11.5)