FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875241
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCAGAGAGGTCAACTGCAGGGAGA79750.42527867084817367No Hit
GTGTAGTACCAGCCACTGGTCTGGCCCGCACAGTAATACACAGCCGTGTC75190.4009617963771057No Hit
GTATTACTGTCAGCATTATGGTACCTCACCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGG60950.3250248901341214No Hit
GTACTACACCGGTCTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCT56130.29932152720636973No Hit
GGCTATGGTGGGTCCCTTAGTGGTTACTACTGGACCTGGATCCGCCAGCC47710.25442063180145913No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43320.23101030747514584No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38840.20712004483690363No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTAAAGTCTGTCCCAGACCCACTGCCA38070.20301390594595572No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCAGAGAGGTCAACTGCAGGGA35180.18760255348512536No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG34940.18632271798664812No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA32180.17160460975415961No Hit
GTGTATTACTGTCAGCATTATGGTACCTCACCCACCTTCGGCCAAGGGAC31250.16664524719756021No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29900.1594461725186256No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29890.1593928460395224No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT29550.15757974575001293No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28920.1542201775665101No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG28590.1524604037561039No Hit
GTATTACTGTGCGGGCCAGACCAGTGGCTGGTACTACACCGGTCTGGACGTCTGGGGCCAAGGGACCACGGTCAC28440.1516605065695556No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC28280.15080728290390408No Hit
CTACTGGACCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTACATTGGGGAAATCAATCCAAGTGGAAGCAC28090.14979407980094292No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA27400.14611455274282079No Hit
GAGTAAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA27020.14408814653689847No Hit
GTGTATTACTGTGCGGGCCAGACCAGTGGCTGGTACTACACCGGTCTGGA26720.14248835216380187No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG26670.1422217197682858No Hit
GTCTCCAGGCGCCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG26600.14184843441456327No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC26570.14168845497725358No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT26130.13934208989671196No Hit
GACTTTACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCATTATGGTACCTCA26110.1392354369385055No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG25870.13795560144002825No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25810.13763564256540892No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25600.13651578650424132No Hit
GTCCCTCACCTGCGCTGGCTATGGTGGGTCCCTTAGTGGTTACTACTGGA25400.13544925692217694No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25120.13395611550728678No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23840.12713032618207473No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23420.12489061405973952No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGTCTGGTACC23410.1248372875806363No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23090.1231308402493333No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23050.12291753433292041No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTAAAGTCTGTCCCAGACCCACTGCCA23010.12270422841650754No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22850.12185100475085602No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22850.12185100475085602No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG22660.12083780164789486No Hit
GTGTATTACTGTGCGGGCCAGACCAGTGGCTGGTACTACACCGGTCTGGACGTCTGGGGCCAAGGGACCACGGT22540.12019788389865624No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22400.11945131319121116No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG22130.11801149825542423No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22000.11731825402708237No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC21990.11726492754797918No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21570.11502521542564395No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGG21280.11347874753165059No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20830.11107905597200574No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC20700.11038581174366388No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20060.10697291708105786No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT19880.1060130404571999No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19730.10521314327065162No Hit
GTCTGGGACAGACTTTACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19610.10457322552141297No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC19210.10244016635728422No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT19200.102386839878181No Hit
GGAGTACATTGGGGAAATCAATCCAAGTGGAAGCACCAACTACAAGCAGT19020.10142696325432304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9950.048.1821141
TATTGAT28500.044.36839770
TAATGTA350.001428688538.96517630
GTATCAA23400.035.5991821
GGGTACG502.028309E-434.000861
TAACGGC851.2808414E-732.25808336
TTAACGG851.292392E-732.22515535
CCGCTAT1153.562595E-430.88664870
TAACCGT952.69647E-729.63218353
TATCAAC28650.028.947062
ATCAACG28650.028.947063
TCAACGC29050.028.665484
CAACGCA29200.028.6346245
CTATTGA28450.028.45304769
TTTATAC605.9181754E-428.3242233
AACGCAG29750.028.1052466
GACCTAT500.00832028227.1912546
TGTTAGA13350.027.13860570
TAGTACC25150.026.6236424
CGCAGAG31350.026.5610128
Produced by FastQC (version 0.11.5)