FastQCFastQC Report
Wed 8 May 2024
EGAF00004689680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875241
Total Bases138.4 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCAGAGAGGTCAACTGCAGGGAGA82320.43898357597770105No Hit
GTGTAGTACCAGCCACTGGTCTGGCCCGCACAGTAATACACAGCCGTGTC81620.43525072244047563No Hit
GTACTACACCGGTCTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCT63180.33691669497413934No Hit
GTATTACTGTCAGCATTATGGTACCTCACCCACCTTCGGCCAAGGGACAC58530.3121198821911424No Hit
GGCTATGGTGGGTCCCTTAGTGGTTACTACTGGACCTGGATCCGCCAGCC53800.2868964575753196No Hit
GTGTATTACTGTGCGGGCCAGACCAGTGGCTGGTACTACACCGGTCTGGA52420.2795374034590754No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT51540.2748446732979921No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47310.25228757263733037No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT43620.23261010184824243No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCAGAGAGGTCAACTGCAGGGA38970.2078132890652455No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37810.20162741748927204No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36140.19272189547903443No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCT34460.1837630469896936No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33840.180456805285294No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA32130.1713379773586435No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32010.17069805960940487No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT31930.17027144777657913No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG31600.16851167396617286No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31560.16829836804976No Hit
GTGTATTACTGTCAGCATTATGGTACCTCACCCACCTTCGGCCAAGGGAC31520.16808506213334712No Hit
GTATTACTGTGCGGGCCAGACCAGTGGCTGGTACTACACCGGTCTGGACG31440.16765845030052137No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA31390.16739181790500526No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT30540.16285906718123164No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA30150.16077933449620607No Hit
GACTTTACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA29980.15987278435145136No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC29840.1591262136440063No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG29660.15816633702014835No Hit
CTACTGGACCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTACATTG29620.1579530311037355No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29580.1577397251873226No Hit
GAGTAAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA29410.15683317504256786No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT28910.15416685108740688No Hit
GTCTCCAGGCGCCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT28740.15326030094265217No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC28630.15267370967251676No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG28540.1521937713605878No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC28000.14931414148901395No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27750.14798097951143346No Hit
GTCCCTCACCTGCGCTGGCTATGGTGGGTCCCTTAGTGGTTACTACTGGA27490.14659449105474978No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA27290.14552796147268537No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTAA26890.14339490230855662No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26440.14099521074891175No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA25480.13587586875500268No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25210.13443605381921578No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG24690.13166307690584836No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGTCTGGTACC24510.13070320028199042No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC24310.12963667069992604No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC24120.12862346759696489No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC23590.12579716420449424No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG23570.12569051124628783No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG22970.12249092250009466No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC22860.12190433122995924No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC22690.12099778108520451No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22580.12041118981506911No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22310.11897137487928218No Hit
GGAGTACATTGGGGAAATCAATCCAAGTGGAAGCACCAACTACAAGCAGT22310.11897137487928218No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT22180.11827813065094032No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT22180.11827813065094032No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21940.11699829515246307No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGG21930.11694496867335985No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21830.11641170388232765No Hit
GTCTGGGACAGACTTTACTCTCACCATCAGCAGACTGGAGCCTGAAGATT21550.11491856246743752No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTC21140.11273217682420551No Hit
GGTCTGGCCCGCACAGTAATACACAGCCGTGTCTGCGGCGGTCAGAGAGG21020.1120922590749669No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC20940.11166564724214113No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG20680.11027915878545744No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC20500.10931928216159949No Hit
GTCCTGTCCCAGGTGCAGCTACAGCAGTGGGGCGCAGGACTGTTGAAGCC20420.10889267032877373No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACC20350.1085193849750512No Hit
GGACTGTTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCGCTGGCTATGG20170.10755950835119325No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20000.10665295820643853No Hit
GTTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCGCTGGCTATGGTGGGT19900.10611969341540635No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC19840.10579973454078702No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT19780.1054797756661677No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG19710.10510649031244518No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG19520.10409328720948399No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG19500.10398663425127758No Hit
GGCCAGACCAGTGGCTGGTACTACACCGGTCTGGACGTCTGGGGCCAAGG19190.10233351339907777No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA19140.10206688100356166No Hit
GCCCCAAGGATGTGCTGGTTCGCTGGCTGCAGGGGTCACAGGAGCTGCCC19070.10169359564983914No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT18920.10089369846329087No Hit
CCTCCATGGTGCATCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTG18810.10030710719315544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)