FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090272
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC56220.5156511402659153No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAG39160.35917642569927505No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC32050.2939633412579613No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31960.2931378591764257No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31930.2928626984825805No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31140.2856168002113234No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGACCATTCAGA31050.2847913181297878No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27000.24764462446068505No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26550.24351721405300697No Hit
GATTATTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTGTTCGG26500.24305861289659828No Hit
GGATGACAGTCTGAATGGTCTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC26270.24094904757711835No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26020.238656041795075No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24360.22343048340230698No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24090.22095403715770007No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24030.22040371577000967No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT23630.21673490651874025No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22310.20462783598955123No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21970.20150934812597227No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21910.2009590267382818No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21900.2008673065070001No Hit
CCCCTGAGGGCCGCTGATTATTTCTATAGATGAGGAGTTTGGGGGCCGATCCTGGGAGTTGCTGGTACCAGTTGA21350.19582269378650463No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20910.1917870036101083No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20820.19096152152857268No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCTGCCTCCCTGGCCATCAGTGGGCTCCAGT20550.18848507528396585No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGACCATTCAGAC19420.17812068914912976No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT19060.1748187608229873No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19030.17454360012914208No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC18930.17362639781632472No Hit
ATAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG18890.17325951689119778No Hit
GTCTGACACCTCTGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG18880.17316779665991605No Hit
GTCTGAGGATGAGGCTGATTATTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTGTTCGGCGGAGGGAC18440.1691321064835197No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17990.16500469607584162No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17900.16417921399430602No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17530.1607855654368818No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCAGAGGTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACC17420.1597766428927827No Hit
CTGTCATCCCATGCAGCACAATAATAATCAGCCTCATCCTCAGACTGGAG17260.15830911919227497No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17230.15803395849842977No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17210.15785051803586628No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17150.15730019664817588No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16880.15482375040356902No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16560.15188870300255347No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16260.14913709606410144No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTATTGTGCTGCATGGGATGACAGTCTGAATGGT15300.14033195386105485No Hit
ATCCTGGGAGTTGCTGGTACCAGTTGACAGTATTCGTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGA15080.1383141087728567No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14950.13712174576619413No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14780.13556250183440463No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC14750.1352873411405594No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14140.12969240703237356No Hit
GCATGGGATGACAGTCTGAATGGTCTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG14110.12941724633852836No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13650.12519811569956854No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13530.12409747292418773No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13430.12318027061137037No Hit
GTATTCGTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT13130.12042866367291832No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13050.11969490182266443No Hit
GTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG13040.1196031815913827No Hit
TTATTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTGTTCGGCG12990.11914458043497402No Hit
CCTCTGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGAT12980.11905286020369228No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12870.11804393765959321No Hit
CTGATGGCCAGGGAGGCAGAGGTGTCAGACTTGGAGCCAGAGAATCGGTC12840.117768776965748No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG12650.11602609257139504No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC12620.11575093187754984No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC12560.11520061048985941No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12400.11373308678935166No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT12310.11290760470781602No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12230.11217384285756216No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12080.11079803938833611No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTATCTG12020.11024771800064571No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11970.10978911684423705No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC11950.10960567638167355No Hit
TTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTGTTCGGCGGAG11940.10951395615039182No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG11870.10887191453141969No Hit
ACACCAGACCATTCAGACTGTCATCCCATGCAGCACAATAATAATCAGCC11800.10822987291244754No Hit
GAACGAATACTGTCAACTGGTACCAGCAACTCCCAGGATCGGCCCCCAAA11700.10731267059963018No Hit
GTCTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC11670.10703750990578498No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAACGAATACTGTCAACTGGTACCAGCAACTCCCAGGATCGGCCCC11610.10648718851809456No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC11570.10612030759296763No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC11400.10456106366117812No Hit
GTCATCCCATGCAGCACAATAATAATCAGCCTCATCCTCAGACTGGAGCC11300.10364386134836079No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG11120.10199289718528955No Hit
GCACAATAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCAGAGGTGTCAGACTTGGAG10970.10061709371606352No Hit
TGGGATGACAGTCTGAATGGTCTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC10950.10043365325350005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)