FastQCFastQC Report
Wed 8 May 2024
EGAF00004689682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090272
Total Bases79.9 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC60000.5503213876904112No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50530.46346232866660797No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAG40450.37100833553461887No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC39660.36376243726336177No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37150.34074065921164626No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT35720.3276246661383581No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGACCATTCAGA35220.3230386545742714No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC34490.3163430776907047No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT33330.3057035308620234No Hit
GGATGACAGTCTGAATGGTCTGGTGTTCGGCGGAGGGACCAAGCTGACCG31870.29231237709489005No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31520.2891021690000294No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC30710.28167283026620876No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC29160.2674561944175398No Hit
GATTATTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTGTTCGG28820.2643377065539609No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC27360.2509465527868275No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26800.24581021983505033No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26740.24525989844735993No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA25880.2373719585571307No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25250.23159358398638138No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG25130.23049294121100056No Hit
GTCTGAGGATGAGGCTGATTATTATTGTGCTGCATGGGATGACAGTCTGA25100.23021778051715536No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA25090.23012606028587362No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT24470.22443940594640602No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT24010.2202202753074462No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCTGCC23980.21994511461360103No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT23960.21976167415103756No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23450.21508394235566905No Hit
CCCCTGAGGGCCGCTGATTATTTCTATAGATGAGGAGTTTGGGGGCCGAT22770.20884696662851104No Hit
GTCTGACACCTCTGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG22170.20334375275160693No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA21760.1995832232690558No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20700.18986087875319185No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20350.18665067065833113No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19910.18261498048193478No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19900.18252326025065305No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT19890.1824315400193713No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19370.17766208799272107No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG18760.17206715388453525No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT18650.17105823134043616No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18450.16922382671480143No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18420.16894866602095623No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18220.16711426139532154No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGACCATTCAGAC18140.16638049954506767No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG17610.1615193272871357No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17320.15885944057996537No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACT17040.15629127410407678No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTATTGTGCTG16630.15253074462152563No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGAC16590.1521638636963987No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAACGAATACTGTCAACTGGT16530.15161354230870827No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA16230.14886193537025624No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGC16210.14867849490769275No Hit
ATCCTGGGAGTTGCTGGTACCAGTTGACAGTATTCGTTCCGATGTTGGAG16190.14849505444512928No Hit
TTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTGTTCGGCGGAG16120.14785301282615712No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG16090.14757785213231192No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCC15970.14647720935693112No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15960.1463854891256494No Hit
CCTCTGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGAT15940.14620204866308592No Hit
GCATGGGATGACAGTCTGAATGGTCTGGTGTTCGGCGGAGGGACCAAGCT15870.14556000704411376No Hit
GAACGAATACTGTCAACTGGTACCAGCAACTCCCAGGATCGGCCCCCAAA15490.14207463825540784No Hit
GTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG15290.14024023362977311No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTG15170.1391395908543923No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC15110.1385892694667019No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC15110.1385892694667019No Hit
GTCTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG15080.1383141087728567No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCTG14920.13684658507234893No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14890.13657142437850373No Hit
CTGTCATCCCATGCAGCACAATAATAATCAGCCTCATCCTCAGACTGGAG14680.13464529952158727No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14590.13381981744005167No Hit
ATAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG14520.1331777758210795No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14120.1295089665698101No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTC14080.12914208564468316No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCT13920.1276745619441754No Hit
TTATTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTGTTCGGCG13750.1261153180123859No Hit
GTATTCGTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT13660.12528983593085027No Hit
CTCCCAGGATCGGCCCCCAAACTCCTCATCTATAGAAATAATCAGCGGCC13640.1251063954682868No Hit
CTGATGGCCAGGGAGGCAGAGGTGTCAGACTTGGAGCCAGAGAATCGGTC13630.12501467523700507No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA13530.12409747292418773No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG13500.12382231223034253No Hit
TGGGATGACAGTCTGAATGGTCTGGTGTTCGGCGGAGGGACCAAGCTGAC13210.12116242552317219No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC12990.11914458043497402No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC12920.11850253881600188No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTC12850.11786049719702973No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT12830.11767705673446625No Hit
GTTTGGGGGCCGATCCTGGGAGTTGCTGGTACCAGTTGACAGTATTCGTT12830.11767705673446625No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC12800.11740189604062105No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCC12660.11611781280267677No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCTGCCTCCCTGGC12620.11575093187754984No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG12560.11520061048985941No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA12530.1149254497960142No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAACGAATACT12440.1140999677144786No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12420.11391652725191513No Hit
GTCTGAATGGTCTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT12340.11318276540166124No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG12220.11208212262628042No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC12120.11116492031346306No Hit
GGATGAGGCTGATTATTATTGTGCTGCATGGGATGACAGTCTGAATGGTC12090.11088975961961786No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12090.11088975961961786No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12050.11052287869449091No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12010.11015599776936398No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG12000.11006427753808225No Hit
GTATATGGTATTTGGACTATTACTAATGTATGAAACCCACTCCAGCCCCT11990.10997255730680051No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11880.10896363476270142No Hit
GCTCCAGTCTGAGGATGAGGCTGATTATTATTGTGCTGCATGGGATGACA11860.10878019430013794No Hit
CTCCTCATCTATAGAAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATT11860.10878019430013794No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA11840.10859675383757447No Hit
GCTTGGTCCCTCCGCCGAACACCAGACCATTCAGACTGTCATCCCATGCA11830.10850503360629274No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG11740.10767955152475714No Hit
GAAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG11550.10593686713040416No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA11510.1055699862052772No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11430.10483622435502334No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC11410.10465278389245987No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11370.10428590296733292No Hit
CCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCC11340.10401074227348772No Hit
ACACCAGACCATTCAGACTGTCATCCCATGCAGCACAATAATAATCAGCC11250.1031852601919521No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11230.10300181972938863No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC11210.10281837926682516No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAA11030.10116741510375392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)