FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536894
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63241.1778861376733583No Hit
GTCTTATATAGTTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAAAA61111.1382135021065611No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22340.41609703218884925No Hit
ATATAAGACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA17790.33135032241001017No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA15670.2918639433482214No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15160.2823648615927911No Hit
GTTTATTACTGTCAACAATATTTTGATATTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA14910.2777084489675802No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13870.2583377724467027No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13300.24772115166122177No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13170.24529981709611207No Hit
ACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAACTATATAAGACAGT11430.21289118522464398No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11300.21046985065953427No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11130.20730349007439083No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10790.20097076890410398No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10170.18942286559358085No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT10140.18886409607855556No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9990.186070248503429No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9920.1847664529683699No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9910.18458019646336146No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCG9750.1816000923832265No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9560.1780612187880662No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTTTGATATT9270.1726597801428215No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9000.16763085450759366No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8500.1583180292571718No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8040.14975023002678367No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8020.1493777170167668No Hit
GCCTTGTACTTCTGCACGACCGGGGGAGTGGTTTGGGGAGTTAAAGTCTA8000.14900520400674994No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7700.14341750885649682No Hit
GTACAAGGCTGTGTCCTCGATTTTCAGGCTGTTCATTTGCAGATACAACG7560.1408099177863787No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7430.138388583221269No Hit
GTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCGGATTCCCGGGT7260.13522222263612557No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC7080.13186960554597368No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6920.1288895014658387No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6770.12609565389071214No Hit
GGCAGGGGCAGCAAGATGGTGTTGCGGACCCAGGTCTTCATTTCTCTGTT6710.1249781148606615No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC6660.12404683233561932No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTCTTATATAGTTCCAC6620.12330180631558557No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6610.12311554981057712No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6580.12255678029555181No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6540.12181175427551807No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6450.12013544573044213No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACC6420.11957667621541682No Hit
CTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATC6320.11771411116533244No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6220.11585154611524807No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6160.11473400708519745No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6080.11324395504512996No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTA5910.11007759445998651No Hit
GGATCTCTGGTGCCTCCGGGGATATCGTTATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG5870.10933256843995277No Hit
GTGTTGCGGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC5860.10914631193494433No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTTTGATATTCCGT5820.10840128591491056No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA5820.10840128591491056No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG5790.10784251639988528No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5750.10709749037985151No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGATGGATTTTC5680.10579369484479245No Hit
GTCTTATATAGTTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTGCT5600.10430364280472496No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5570.10374487328969964No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT5540.10318610377467433No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5440.10132353872458995No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5390.10039225619954777No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC5380.10020599969453932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATCG100.0071112397144.8391970
TACACGG200.00734926351.76341641
AAGGTCG200.00813790550.44592716
AATGGTA1501.0913936E-1148.2797370
TTGAACG306.3538796E-445.93282740
GTGGTAT2050.045.8959921
GTTATAT307.183328E-444.8032261
TGGTATC2150.043.7735252
ACAGGCC705.7298166E-1043.2031141
GACAGGT858.731149E-1139.532261
TTGAGAT3000.038.1716469
GTATCAA15000.036.7386471
GGTATCA5950.036.7085271
TTGCTCA28400.036.46479470
ACGCTTT400.002508908334.73825544
CACCAAT16150.033.7315915
ACCAATA16200.033.63061516
TAGGAAC16100.033.44043721
CCACCAA16700.033.01108614
CCAATAG16650.032.52272817
Produced by FastQC (version 0.11.5)