FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881148
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28150.3194696010204869No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25200.28599054869329554No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT23150.26272544453372193No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGA22980.2607961432131719No Hit
ACTTACTACTGTCAACAGACTTACGGTCCCCCCACTTTCGGCGGGGGGAC20250.22981383377139822No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19090.21664918946646874No Hit
GATCTATGCTGCATCCTATTTGCATGGAGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGGCAGATTTCA18820.2135850050161834No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18420.20904547249724223No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18330.20802407768048045No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17590.19962594252043922No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAACTATTTAAATTGGTATCAGCAGAAA16720.18975245929174214No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15430.17511246691815677No Hit
GTAGTAAGTTGCACAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCACTGCCA15140.1718213058419244No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14890.16898409801758615No Hit
GTCTCTGGTGGCTCCATTAGTAGTTACTCCTGGAGCTGGATCCGGCAGCC14770.16762223826190378No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAA14400.1634231706818832No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14250.16172084598728023No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14090.15990503297970374No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14050.15945107972780964No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14040.15933759141483608No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13960.15842968491104786No Hit
GCATAGATCAGGATCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT13760.15615991865157725No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13420.15230131601047725No Hit
GACTTACGGTCCCCCCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT13330.15127992119371547No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13280.15071247962884782No Hit
ATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCACTGCCACTGAACCTTGATGGGAC13060.14821573674343017No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12910.1465134120488272No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12150.13788830026283894No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11890.13493760412552716No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11530.13085202485848008No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCGA11360.12892272353793008No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11330.12858225859900949No Hit
CCTGAAGATTGTGCAACTTACTACTGTCAACAGACTTACGGTCCCCCCAC10980.12461016764493593No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10940.12415621439304181No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10910.12381574945412122No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10440.1184817987443653No Hit
CCGTAAGTCTGTTGACAGTAGTAAGTTGCACAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGCC10160.11530412598110647No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC10140.1150771493551594No Hit
GTGTATTACTGTGCGAGACGGGGACGGGGTGAGATGCCATTTGTGTACTACTTTGACTTATGGGGCCAGGGAACC9890.11223994153082115No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC9790.11110505840108585No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9780.11099157008811232No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9400.10667901419511819No Hit
CTATTTGCATGGAGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGG9250.10497668950051524No Hit
GATCAGGATCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT9150.10384180637077994No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9010.10225296998915051No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8970.1017990167372564No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA8820.10009669204265345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATT200.00796292950.72681823
TCGTATG4650.041.49751740
GTATCAA22450.041.1237451
CGTATGC4700.041.1226841
ACCGTAC4650.040.7871327
CGTACTA4700.039.69358429
ATCTCGT4000.039.4052537
GATCTCG4000.039.33960336
CTAGATC4000.039.1708433
GTATGCC4950.039.11605542
CACGAGA4850.039.03857821
GCCGTCT5000.039.0148546
ATGCCGT5000.038.87159744
GTGGTAT2100.038.6490021
CTTATAC5000.038.552381
GTACTAG4900.038.1059930
TACTAGA4850.037.8333731
ACTAGAT4500.037.807332
CCGTCTT5250.037.213647
GACAGGT2000.037.1996651
Produced by FastQC (version 0.11.5)