FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881148
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51070.5795848143558177No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28050.3183347178907516No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGA21700.2462696391525601No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21660.24581568590066596No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19780.2244798830616423No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19550.2218696518632511No Hit
ACTTACTACTGTCAACAGACTTACGGTCCCCCCACTTTCGGCGGGGGGAC18990.21551430633673344No Hit
GATCTATGCTGCATCCTATTTGCATGGAGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGGCAGATTTCA18530.21029384393995107No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17200.19519989831447157No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAACTATTTAAATTGGTATCAGCAGAAA16100.18271618388738328No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15580.17681479161275973No Hit
GTAGTAAGTTGCACAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCACTGCCA15290.17352363053652733No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14450.16399061224675082No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14340.16274224080404198No Hit
GTCTCTGGTGGCTCCATTAGTAGTTACTCCTGGAGCTGGATCCGGCAGCC14090.15990503297970374No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13810.1567273602164449No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAA13810.1567273602164449No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13390.15196085107155666No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13350.15150689781966253No Hit
ATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCACTGCCACTGAACCTTGATGGGAC13230.15014503806398016No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13080.14844271336937723No Hit
GACTTACGGTCCCCCCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT13060.14821573674343017No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12680.14390318085043602No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12560.14254132109475368No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12360.14027155483528309No Hit
GCATAGATCAGGATCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT12110.1374343470109448No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12060.13686690544607716No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11770.1335757443698448No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11760.13346225605687126No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC11620.13187341967524185No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC11230.12744737546927418No Hit
GTGTATTACTGTGCGAGACGGGGACGGGGTGAGATGCCATTTGTGTACTACTTTGACTTATGGGGCCAGGGAACC11170.126766445591433No Hit
CCTGAAGATTGTGCAACTTACTACTGTCAACAGACTTACGGTCCCCCCAC10940.12415621439304181No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCGA10710.12154598319465061No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10290.11677947404976236No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT10130.11496366104218587No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10100.1146231961032653No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC9690.10997017527135054No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9390.10656552588214464No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9330.10588459600430347No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9260.10509017781348876No Hit
CCGTAAGTCTGTTGACAGTAGTAAGTTGCACAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGCC9180.10418227130970052No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9120.10350134143185935No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8840.10032366866860051No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)