FastQCFastQC Report
Wed 8 May 2024
EGAF00004689688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771418
Total Bases56.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104981.3608705008179742No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39110.5069884291006951No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT38940.5047846951976749No Hit
CTATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGG38730.5020624356704148No Hit
CTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT38210.4953216025552942No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30010.38902384958608693No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28410.3682828246164855No Hit
GAGTATCAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGCAAAGCCC27130.35169000464080435No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26960.34948627073778415No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA25120.3256340920227425No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24870.3223933068712423No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTGCCTGGTTTCTGCTGATACCAG24490.31746731344096196No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT24100.3124116886046216No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21830.2829853594289996No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21120.2737815295987389No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20980.27196668991389883No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20960.2717074271017788No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20380.2641888055502983No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC19720.2556331327503377No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19620.25433681868973756No Hit
TCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCATCAAGGTTCAGCGGC19610.2542071872836776No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG19400.2514849277564174No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA19100.24759598557461712No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC17810.23087353419287598No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC17520.22711422341713572No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17130.22205859858079538No Hit
GAATTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA16890.21894744483535516No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16710.21661407952627498No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA16430.21298440015659476No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG16400.2125955059384147No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15920.2063731984475343No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15240.1975582628354537No Hit
GGCCAAAAGAGTACCCCGGAGAATAACTATTATAGTGTAGGCAGTAATAA15130.19613231736879355No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATCAGTAG15080.19548416033849356No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGAG14480.18770627597489298No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACTCCA14070.18239138832643262No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14070.18239138832643262No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGA13840.1794098659870524No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13820.1791506031749324No Hit
ACTTATTACTGCCTACACTATAATAGTTATTCTCCGGGGTACTCTTTTGG13800.17889134036281237No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT13700.17759502630221227No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA13560.17578018661737219No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA13210.17124308740527186No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC12720.16489114850833142No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC12620.16359483444773132No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT12580.16307630882349128No Hit
CTGATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA12330.15983552367199108No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12280.159187366641691No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12120.15711326414473087No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11550.14972427399931038No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA11480.1488168541568903No Hit
ACCCAGTCTCCTGCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCAT11400.14777980290841022No Hit
GTTTAGGGGCTTTGCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTG11340.14700201447205016No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11220.14544643759933007No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11130.14427975494479No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT11050.1432427036963099No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10920.1415574954175298No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10810.1401315499508697No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG10700.1387056044842096No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC10590.1372796590175495No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10590.1372796590175495No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10580.1371500276114895No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC10500.13611297636300942No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10250.1328721912115092No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9860.12781656637516886No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9640.12496467544184865No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9560.12392762419336859No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9440.12237204732064846No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9400.12185352169640842No Hit
ATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAG9370.12146462747822839No Hit
GGTATCAGCAGAAACCAGGCAAAGCCCCTAAACTCCTGATCTATAAGGTG9310.12068683904186836No Hit
ATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC9060.11744605389036812No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA9030.11705715967218809No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT8900.11537195139340799No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA8870.11498305717522796No Hit
GTCTCCTGCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT8780.11381637452068788No Hit
CACTATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCT8520.11044595796312764No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8440.10940890671464758No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT8360.10837185546616748No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8340.10811259265404748No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8340.10811259265404748No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8330.10798296124798748No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG8290.10746443562374744No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8290.10746443562374744No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8280.10733480421768742No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC8240.10681627859344739No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8240.10681627859344739No Hit
GATGCAGACAGGGTGGCAGGAGACTGGGTCATCTGGATGTCACATTTGGC8140.10551996453284732No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC8110.10513107031466729No Hit
GTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG8110.10513107031466729No Hit
CCTGCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG7950.10305696781770714No Hit
ACCTTGATGGGACTCCACTTTCTAAATTAGACACCTTATAGATCAGGAGT7940.10292733641164711No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT7920.10266807359952711No Hit
TAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCA7890.10227917938134708No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7870.10201991656922706No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7860.10189028516316705No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7830.10150139094498702No Hit
CTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCATCAAGGTT7800.10111249672680701No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA7730.10020507688438694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)