FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033531
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35590.34435348334979793No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32100.3105857492421611No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29890.28920274283016184No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG27150.26269168510668767No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23890.23114933175686073No Hit
CCCCTATACTTTTGGCCAGGGGACCAAGCTTGAGATCAAACGAACTGTGG23470.22708559298172962No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23410.2265050588709966No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23110.22360238831733154No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22670.2193451381719561No Hit
GTATAGGGGAGATAATTATATTGTTGACAGTAATATGTTGCAAAATCGTC21880.21170143904730485No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21390.20696041047631855No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20920.20241289327557663No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19890.19244705770799328No Hit
GTAATATGTTGCAAAATCGTCAGGCTGCAGGCCGGTGATGGTGAAAGTAA19660.1902216769501834No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18830.1821909550850434No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17930.17348294342404824No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17580.17009649444477234No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17380.16816138074232898No Hit
GTCTCCATCCTCCCTGGCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCAGGCGAGTCAGGCCATTAGTTA17150.16593599998451908No Hit
CAGTAATATGTTGCAAAATCGTCAGGCTGCAGGCCGGTGATGGTGAAAGTAAAGTGTGTCCCAGATCCACTGGCA17120.16564573292915258No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16100.1557766530466914No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT15730.15219669269717115No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14970.14484326062788633No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14900.14416597083203114No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14840.14358543672129814No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14710.14232761281470996No Hit
CTAATGGCCTGACTCGCCTGGCAAGTGATGGTGATTCTGTCTCCTACAGA14610.14136005596348827No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14460.13990872068665575No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14450.1398119650015336No Hit
GTTTAGGGGCTCTCCCTGATTTGTGTTGATACCAACTTAAATAGTAACTA14080.13623200465201335No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13350.12916883963809506No Hit
GGCCATTAGTTACTATTTAAGTTGGTATCAACACAAATCAGGGAGAGCCCCTAAACTCCTGATCTCCAGGGCATC13220.12791101573150684No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13110.12684670319516297No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13010.12587914634394132No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12890.12471807812247528No Hit
ATATTACTGTCAACAATATAATTATCTCCCCTATACTTTTGGCCAGGGGA12610.12200891893905458No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT12600.12191216325393239No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12560.12152514051344372No Hit
CACTTTACTTTCACCATCACCGGCCTGCAGCCTGACGATTTTGCAACATATTACTGTCAACAATATAATTATCTC12370.11968678249612251No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC12140.11746140173831264No Hit
GTATTAACTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGATGGGTCAGCGCTTTCAAGCAAAGCA11960.11571979940611361No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11870.11484899824001407No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11680.11301064022269289No Hit
ATATAATTATCTCCCCTATACTTTTGGCCAGGGGACCAAGCTTGAGATCAAACGAACTGTGGCTGCACCAT11610.1123333504268377No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11390.11020472535414999No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11370.11001121398390566No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT11340.10972094692853916No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11130.10768907754097361No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11120.10759232185585145No Hit
CTATTTAAGTTGGTATCAACACAAATCAGGGAGAGCCCCTAAACTCCTGA10860.10507667404267505No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10820.1046896513021864No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10810.10459289561706422No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10760.10410911719145337No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10740.10391560582120904No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10730.10381885013608687No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10670.10323831602535385No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10560.10217400348901001No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT10540.10198049211876568No Hit
ACCTTGATGGGATCCCTGTTTCCAAAGTGGATGCCCTGGAGATCAGGAGT10530.1018837364336435No Hit
ACATATTACTGTCAACAATATAATTATCTCCCCTATACTTTTGGCCAGGGGACCAAGCTTGAGATCAAACGAA10520.10178698074852133No Hit
GTAGGAGACAGAATCACCATCACTTGCCAGGCGAGTCAGGCCATTAGTTA10470.10130320232291048No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10410.10072266821217749No Hit
GTGCAGCCACAGTTCGTTTGATCTCAAGCTTGGTCCCCTGGCCAAAAGTA10350.10014213410144447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA200.005772945555.02492563
TAGTTCG200.00805888650.573959
GTGGTAT2100.043.376381
CTTATAC5000.036.4361571
CCCACGA5000.036.4220519
CGTATGC3650.035.9360641
TCGTATG3400.035.44322640
TATACAC5550.035.236413
GATCTCG3600.035.11287736
CACGAGA5200.035.03137621
CCGTCGT601.231663E-534.93628347
ATCTCGT3350.034.73167837
CTCTTAT2550.034.3986971
CGAGACA5350.034.05578223
TCTTATA2400.033.7175982
GTATGCC4300.032.977442
ATCTCCG5650.032.82402810
TTATACA5550.032.806312
AATGGTA4850.032.70588370
ATGCCGT4700.032.53505744
Produced by FastQC (version 0.11.5)