FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033531
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49130.4753606810052141No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32490.31435922096192565No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29510.28552602679551947No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29440.28484873699966423No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG28320.2740121002659814No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25390.24566268452518597No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22580.2184743370058566No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22560.2182808256356123No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21260.2057025865697304No Hit
GTATAGGGGAGATAATTATATTGTTGACAGTAATATGTTGCAAAATCGTC20560.19892968861117857No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20410.19747835333434605No Hit
CCCCTATACTTTTGGCCAGGGGACCAAGCTTGAGATCAAACGAACTGTGG20090.19438217141043665No Hit
GTAATATGTTGCAAAATCGTCAGGCTGCAGGCCGGTGATGGTGAAAGTAA19270.18644820523041883No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18790.18180393234455472No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18490.17890126179088967No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17950.17367645479429258No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17260.16700031252086295No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17200.16641977841012995No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16980.16429115333744224No Hit
CAGTAATATGTTGCAAAATCGTCAGGCTGCAGGCCGGTGATGGTGAAAGTAAAGTGTGTCCCAGATCCACTGGCA16870.1632268408010984No Hit
GTCTCCATCCTCCCTGGCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCAGGCGAGTCAGGCCATTAGTTA16840.16293657374573187No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16510.15974363613670028No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16070.1554863859913249No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15540.15035833467984996No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14610.14136005596348827No Hit
GTTTAGGGGCTCTCCCTGATTTGTGTTGATACCAACTTAAATAGTAACTA14370.13903791952055625No Hit
CTAATGGCCTGACTCGCCTGGCAAGTGATGGTGATTCTGTCTCCTACAGA14030.1357482262264025No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13820.13371635683883695No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13590.13149097608102708No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13500.13062017491492758No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13380.12945910669346156No Hit
GGCCATTAGTTACTATTTAAGTTGGTATCAACACAAATCAGGGAGAGCCCCTAAACTCCTGATCTCCAGGGCATC13190.12762074867614034No Hit
ATATTACTGTCAACAATATAATTATCTCCCCTATACTTTTGGCCAGGGGA13170.127427237305896No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13020.12597590202906347No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT12790.1237505212712536No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12790.1237505212712536No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12670.12258945304978758No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12540.1213316291431994No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12220.11823544721928998No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12020.11630033351684663No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11770.11388144138879241No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11690.11310739590781504No Hit
ACATATTACTGTCAACAATATAATTATCTCCCCTATACTTTTGGCCAGGGGACCAAGCTTGAGATCAAACGAA11580.1120430833714712No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11560.11184957200122687No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11540.11165606063098252No Hit
ATATAATTATCTCCCCTATACTTTTGGCCAGGGGACCAAGCTTGAGATCAAACGAACTGTGGCTGCACCAT11530.11155930494586036No Hit
GTATTAACTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGATGGGTCAGCGCTTTCAAGCAAAGCA11380.11010796966902782No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11310.10943067987317266No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11160.10797934459634011No Hit
CACTTTACTTTCACCATCACCGGCCTGCAGCCTGACGATTTTGCAACATATTACTGTCAACAATATAATTATCTC11160.10797934459634011No Hit
GTGCAGCCACAGTTCGTTTGATCTCAAGCTTGGTCCCCTGGCCAAAAGTA11070.1071085434302406No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10780.10430262856169772No Hit
TAATTATATTGTTGACAGTAATATGTTGCAAAATCGTCAGGCTGCAGGCCGGTGATGGTGAAAGTAAAGTGTGTC10730.10381885013608687No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC10690.1034318273955982No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC10580.10236751485925434No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT10480.10139995800803267No Hit
ACCTTGATGGGATCCCTGTTTCCAAAGTGGATGCCCTGGAGATCAGGAGT10420.10081942389729964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)