FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875307
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT67780.7743568827851256No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25510.29144060312553194No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24300.2776168818483115No Hit
GTTTATTACTGTCAGCAATATTATCTTTCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA18160.20747006478869698No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC17170.1961597473800621No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16690.19067595712133No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16510.18861953577430549No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15160.1731963756716215No Hit
CCTTAGGAGGCTGTCCTGGTCTTTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGCCACTAAATAAAAGACT15140.172967884410841No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14350.16394247961001113No Hit
GTAGTACACGGCTGTGTCCTCTGTCTTCAGGTTGTTCATTTGCAGATATA13760.15720198741698627No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13690.1564022680042545No Hit
CTCCTAAGGTACTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT13650.15594528548269349No Hit
GTAATAAACAGCCGCATCTTCAGCCTGCAGCCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCA13500.15423160102683972No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12990.1484050738769369No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12790.14612016126913185No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12500.14280703798781458No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12230.13972240596727775No Hit
GTCTCCAGGCTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT12190.13926542344571677No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12050.13766598462025326No Hit
ATATTATCTTTCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT11960.13663777394674095No Hit
GACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTG11770.1344671069693262No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11190.1278408604066916No Hit
GTGTACTACTGTGCCACAGTCAAGGCATATTATGAGAGTAGTGGCTATTTCTCAAATTGGTTCGACCCCTGGGGC10960.1252132109077158No Hit
CCCTTAGACTCTCTTGTGCAGGCTCAGGTTTCACTTTCAGAAACGCCTGG10810.12349952645186203No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10770.12304254393030102No Hit
CTCCAGGCTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC10270.11733026241078845No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10070.11504534980298341No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10040.11470261291181266No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC9820.11218920904322713No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9750.11138948963049534No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9680.11058977021776359No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9630.11001854206581234No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9560.10921882265308058No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9500.10853334887073907No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG9410.1075051381972268No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9300.10624843626293404No Hit
ATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGGC9300.10624843626293404No Hit
CAATAAGAACTACTTAGCTTGGTACCAACAAAGACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCTTCTAC9280.10601994500215355No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAC9250.10567720811098277No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9240.10556296248059253No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9080.10373503239434849No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9060.10350654113356798No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9020.10304955861200697No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGGTGGATTTTC8990.10270682172083623No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8940.10213559356888498No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8810.1006504003738117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTT150.002668257366.8630114
TTCGTAT400.006679220653.02813370
CGCGTAT352.3341783E-549.06258439
GTCGTAT451.9198924E-645.79174439
CTTATAC15200.044.006421
TATGTCG551.633307E-743.32132736
AGGGGGG17850.043.17510270
GCGTATG405.032565E-543.0789240
CTAGCTC950.042.90078435
TTATACA15750.042.662532
TATACAC15750.042.662533
CGTATGC14600.042.62938341
ATGCCGT14750.042.3114344
TCGTATG14500.042.30646540
CCGTCTT14400.042.28005247
GCCGTCT14300.041.94478246
GTATGCC15050.041.9108242
GCTTGAA14650.041.7062156
CGTCTTC14700.041.04699348
GACGGAC15600.041.0376626
Produced by FastQC (version 0.11.5)