FastQCFastQC Report
Wed 8 May 2024
EGAF00004689692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875307
Total Bases63.6 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG152201.73881849453963No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29730.3396522591502181No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27660.31600341365943607No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21010.2400300694499187No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20320.23214712095299136No Hit
GTTTATTACTGTCAGCAATATTATCTTTCTCCGCTCACTTTCGGCGGAGG20170.2304334364971376No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19580.2236929443041127No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17410.1989016425094281No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT17170.1961597473800621No Hit
GTAGTACACGGCTGTGTCCTCTGTCTTCAGGTTGTTCATTTGCAGATATA16090.1838212192979149No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15700.1793656397126951No Hit
GTAATAAACAGCCGCATCTTCAGCCTGCAGCCTGCTGATGGTGAGAGTGA15290.17468156886669478No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15260.17433883197552402No Hit
CCTTAGGAGGCTGTCCTGGTCTTTGTTGGTACCAAGCTAAGTAGTTCTTA15250.17422458634513377No Hit
CTCCTAAGGTACTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT15210.17376760382357276No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14750.16851230482562118No Hit
GACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCTTCTACCCGGGAA14670.16759833978249916No Hit
ATATTATCTTTCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA13990.15982963691596205No Hit
GTCTCCAGGCTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT13620.15560254859152275No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC13360.1526321622013762No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC13340.1524036709405957No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13330.15228942531020545No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC13330.15228942531020545No Hit
CTCCAGGCTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC13220.15103272337591267No Hit
GTGTACTACTGTGCCACAGTCAAGGCATATTATGAGAGTAGTGGCTATTT13120.14989026707201017No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12900.1473768632034246No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12560.14349251177015607No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA12070.13789447588103376No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA12070.13789447588103376No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11740.13412437007815542No Hit
CCCTTAGACTCTCTTGTGCAGGCTCAGGTTTCACTTTCAGAAACGCCTGG11340.1295545448625454No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11330.1294402992321551No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11190.1278408604066916No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG10990.12555594779888657No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10760.12292829829991078No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA10670.1219000876263985No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC10620.12132885947444726No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC10510.12007215754015449No Hit
CAATAAGAACTACTTAGCTTGGTACCAACAAAGACCAGGACAGCCTCCTA10490.11984366627937398No Hit
GTCCTGGTCTTTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGCCACTA10370.11847271871469095No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGGTGGATTTTC10250.11710177115000794No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10210.11664478862844693No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10200.11653054299805667No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10000.11424563039025165No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAC9930.1134459109775199No Hit
ATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGGC9770.11161798089127585No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT9750.11138948963049534No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9640.11013278769620258No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9570.10933306828347082No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9560.10921882265308058No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9480.10830485760995856No Hit
GATTTCACTCTCACCATCAGCAGGCTGCAGGCTGAAGATGCGGCTGTTTA9290.10613419063254378No Hit
CTCTTGTGCAGGCTCAGGTTTCACTTTCAGAAACGCCTGGATGAATTGGG9290.10613419063254378No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9050.10339229550317774No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9010.10293531298161673No Hit
GTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT8830.10087889163459221No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8790.1004219091130312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)