FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029565
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67240.33130252049084413No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63040.31060843087065454No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43910.21635177981488646No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42820.21098117084202775No Hit
CCCTTGGGCAGCCGGCCTCCATCTCCTGCTGGTCTAGCCAGAGCCTCGTG42060.20723652605361248No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40950.2017673737968481No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG40500.19955014990897063No Hit
GTGTGTGCCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC38150.18797131405005504No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGGGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC36850.18156600059618688No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35500.1749143289325545No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34610.17052915279875244No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33450.1648136423322239No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33410.1646165557644126No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG32660.16092118261795016No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32490.16008356470475202No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32250.158901045297884No Hit
GTCCCAGAGAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA32120.1582605139524972No Hit
CTCTGGGACCCCGGAGTCCCGGTTAGAAACCCTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA31080.15313626318940265No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30830.15190447214058186No Hit
GGCACACACTGGCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT30540.1504755945239497No Hit
GGTCTAGCCAGAGCCTCGTGTACAGTGATGGAAACACGTACTTAAATTGGTTTCAGCAGAGGCCAGGCCAATCTC29800.14682949301944012No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29550.14559770197061933No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT29370.14471081241546835No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC28220.13904457359089265No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA28140.13865040045526997No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27860.13727079448059065No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAGGGTTTCTAACCGGGAC27480.13539847208638303No Hit
CAATGGGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA27280.1344130392473264No Hit
GGACTCCTCAGTTCACCTTCTCACAATGGGGCTCCCTGCTCAGCTCCTGG27250.1342652243214679No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGGCAGCCGGCCTCCATCTCCTGCTGGTCTAGCCAGAGCCTCGTGTA27040.13323051984045842No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT26840.13224508700140178No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26790.13199872879163763No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC26590.131013295952581No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG26520.13066839445891115No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26400.13007713475547716No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCC25770.12697302131244873No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25520.1257412302636279No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG24550.12096188099420319No Hit
GTGATGGAAACACGTACTTAAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAGGGTTT23930.1179070391931276No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23490.11573908694720296No Hit
GTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23330.11495074067595765No Hit
AGCCAGAGCCTCGTGTACAGTGATGGAAACACGTACTTAAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGG23250.11455656754033501No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22400.11036847797434425No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22200.10938304513528761No Hit
CTATAGTAGAAGTAACTGTCCCATATACAATAATACACGCCTGTGTCCTC22190.10933377349333477No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22070.1087425137899008No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22060.10869324214794797No Hit
CTCGTGTACAGTGATGGAAACACGTACTTAAATTGGTTTCAGCAGAGGCC21880.10780635259279697No Hit
CGTGTACAGTGATGGAAACACGTACTTAAATTGGTTTCAGCAGAGGCCAG21840.10760926602498566No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21730.1070672779635045No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21730.1070672779635045No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21680.10682091975374033No Hit
CCCTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAACCAA21650.10667310482788185No Hit
GTTTATTACTGCATGCAAGGCACACACTGGCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAA21490.10588475855663652No Hit
CCTCAGTTCACCTTCTCACAATGGGGCTCCCTGCTCAGCTCCTGGGGCTG21480.10583548691468368No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21240.1046529675078157No Hit
CTCCCTGCCCGTCACCCTTGGGCAGCCGGCCTCCATCTCCTGCTGGTCTA21210.10450515258195721No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21210.10450515258195721No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA21200.10445588094000438No Hit
CAATAATACACGCCTGTGTCCTCGGTTGTCAGGCTGTTCATTTGCAGATA21150.10420952273024021No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21080.1038646212365704No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21030.10361826302680624No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG20730.10214011376822127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)