FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029565
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66230.326326084653608No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47880.23591262167016086No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44820.22083549923259418No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG41840.2061525499306502No Hit
GTGTGTGCCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC41720.20556129022721614No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41700.20546274694331051No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41280.20339333798129156No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37800.18624680658170595No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGGGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC36410.17939804835026224No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33200.16358185128340308No Hit
CCCTTGGGCAGCCGGCCTCCATCTCCTGCTGGTCTAGCCAGAGCCTCGTG33150.16333549307363893No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32880.16200515874091245No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31650.1559447467807141No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30930.15239718856011017No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30660.15106685422738372No Hit
CTCTGGGACCCCGGAGTCCCGGTTAGAAACCCTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA30270.14914526019122323No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29930.14747002436482695No Hit
GTCCCAGAGAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA28540.14062126613338324No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28300.1394387467265153No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27780.136876621344968No Hit
GGCACACACTGGCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT27330.13465939745709057No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27220.1341174093956094No Hit
GGTCTAGCCAGAGCCTCGTGTACAGTGATGGAAACACGTACTTAAATTGGTTTCAGCAGAGGCCAGGCCAATCTC27100.1335261496921754No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGGCAGCCGGCCTCCATCTCCTGCTGGTCTAGCCAGAGCCTCGTGTA26440.13027422132328847No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC26410.13012640639743No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25820.12721937952221288No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG25680.12652957653487323No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT25670.12648030489292042No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC25440.12534705712800526No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25380.12505142727628826No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCC25020.12327764816598631No Hit
CAATGGGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA24890.1226371168205995No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAGGGTTTCTAACCGGGAC24680.12160241233959No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC24450.12046916457467488No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24080.11864611382242007No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23830.11741432277359927No Hit
CTCGTGTACAGTGATGGAAACACGTACTTAAATTGGTTTCAGCAGAGGCC23770.11711869292188229No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23330.11495074067595765No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23110.11386676455299534No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22650.11160026902316506No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22590.11130463917144806No Hit
GGACTCCTCAGTTCACCTTCTCACAATGGGGCTCCCTGCTCAGCTCCTGG22580.11125536752949525No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22540.1110582809616839No Hit
GTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTG22220.10948158841919328No Hit
CCCTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAACCAA21730.1070672779635045No Hit
GTGATGGAAACACGTACTTAAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAGGGTTT21490.10588475855663652No Hit
CTCCCTGCCCGTCACCCTTGGGCAGCCGGCCTCCATCTCCTGCTGGTCTA21220.10455442422391005No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20870.10282991675556093No Hit
GTTTATTACTGCATGCAAGGCACACACTGGCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAA20840.10268210182970243No Hit
CCTCAGTTCACCTTCTCACAATGGGGCTCCCTGCTCAGCTCCTGGGGCTG20820.10258355854579676No Hit
CGTGTACAGTGATGGAAACACGTACTTAAATTGGTTTCAGCAGAGGCCAG20350.10026779137401365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT200.00781622250.9684219
GTGGTAT4200.049.319961
GACTAGT657.735388E-444.13826870
GGTATCA16200.041.294611
GTACGGT350.001457875238.805323
TCGGTAG750.001563827638.25316270
GTATCAA40400.038.160731
TGGTATC5250.038.1595042
TATCAAC47250.032.4822922
ATCAACG47300.032.375373
TCAACGC47900.032.0383534
AACGCAG48450.031.9541726
CAACGCA48500.031.710445
ACGCAGA51000.030.2898947
CGCAGAG51550.030.0325838
AGAGTAC52050.029.8730711
CAGAGTA53000.029.33833110
GCAGAGT53350.029.01939
GAGTTTA6700.028.382811
GCTTGTA1203.1832315E-928.2983361
Produced by FastQC (version 0.11.5)