FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641253
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA98680.6012479489755693No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC95390.5812022887391524No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG87230.5314841770281609No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC86050.5242945481287773No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGAGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG77400.47159091255278746No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG61790.37648065228212835No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT61750.3762369360482509No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG58780.3581410056828533No Hit
GTAATAGTCAGCCTCATCGTCAGACTGGAGGCTGGAGATGGTGAGGTAGC55420.3376688420371509No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG54970.33492703440603006No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT53800.32779833456511576No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT53360.3251174559924643No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT53270.3245690944662401No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC52960.32268029365369016No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC52900.3223147193028741No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG50330.3066559512762505No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC50130.30543737010686345No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT49790.3033657821189055No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC49330.3005630454293153No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG49060.29891796085064276No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC49020.2986742446167654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48810.29739473438890895No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG48620.2962370822779913No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA47900.2918501900681979No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG46860.28551356798738525No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTACTGTCAGAC45730.27862858438034843No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA45730.27862858438034843No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT45510.27728814509402266No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG45300.2760086348661663No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC44060.2684534316159666No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT42660.25992336343025724No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG41450.25255094735546557No Hit
CCCAAGAGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCGTCA41240.2512714371276092No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA41130.2506012174844463No Hit
CTATTACTGTCAGACCTGGGGCACTGGCTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC40360.2459096799823062No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC39710.2419492911817983No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG39350.2397558450769016No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37140.22629052315517473No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA36250.22086783695140239No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA36160.2203194754251782No Hit
CAGTAATAGTCAGCCTCATCGTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC35440.2159325832153848No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35350.21538422168916066No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGAGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGC34550.21050989701161246No Hit
ACTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT34220.20849923808212387No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC33900.20654950821110454No Hit
CTGTTAAGTTTCATCAAGTATCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC33720.20545278515865623No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA33690.20526999798324816No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC33650.20502628174937074No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT33330.20307655187835147No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC33170.2021016869428418No Hit
GGCTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT32630.1988115177854968No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC31660.19290139911396964No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC31250.19040330771672617No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC31020.18900193937193108No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCT30740.1872959257347892No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG29860.18193416858948622No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT29810.18162952329713944No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA29700.18095930365397658No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC29410.17919236095836535No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC29210.1779737797889783No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG28580.1741352491054091No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT28280.17230737735132853No Hit
CTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAG28220.17194180300051243No Hit
CTACCTCACCATCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTACT28210.17188087394204304No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGAGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCGTCAGA27690.16871256290163675No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC27380.1668237620890868No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG27130.165300535627353No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT26580.1619494374115386No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA26200.15963413318970324No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTG26160.15939041695582581No Hit
GTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGG25860.15756254520174526No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC25860.15756254520174526No Hit
CCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCT25770.15701418367552109No Hit
GTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTG25680.1564658221492969No Hit
GGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGG25370.154577021336747No Hit
GAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCTGTGGCT25120.1530537948750132No Hit
GAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGG24100.14683903091113923No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC24090.14677810185266987No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGGCACAGC23970.14604695315103766No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23710.1444627976308345No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC23390.14251306775981523No Hit
CCCTTGCTGTGGCTGCCATCACTGTTAAGTTTCATCAAGTATCGAGGGCC23350.1422693515259378No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA22990.14007590542104112No Hit
GCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAG22950.13983218918716372No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC22810.13897918236859277No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC22520.13721223967298157No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG22310.13593272944512516No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGC22190.13520158074349292No Hit
GAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAG22130.13483600639267682No Hit
GAGAAGGGCCCTCGATACTTGATGAAACTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCG22020.13416578674951393No Hit
GTCTGACGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCTCTTGGGTGTTCGGCGGAGGGACCAAG21620.13172862441073985No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC21570.1314239791183931No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT21330.12996168171512862No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20990.12789009372717064No Hit
CTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCC20860.12709801596706907No Hit
TCGTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC20780.12661058349931423No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC20710.12618408009002877No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC20580.1253920023299272No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC20130.12265019469880634No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG20130.12265019469880634No Hit
GACCTGGGGCACTGGCTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC19840.12088325200319512No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC19700.1200302451846242No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA19530.1189944511906452No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG19360.1179586571966662No Hit
CAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGGCCCTC19110.1164354307349324No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18840.11479034615625987No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG18710.1139982683961583No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC18650.1136326940453422No Hit
CTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATC18650.1136326940453422No Hit
CCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAA18540.1129624744021793No Hit
GGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCG18490.11265782910983253No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18460.1124750419344245No Hit
GCTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC18330.1116829641743229No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18300.11150017699891486No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA18260.11125646076503744No Hit
ACCTCACCATCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTACTGT18050.10997695053718104No Hit
GTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAG17530.10680863949677473No Hit
TACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT17350.10571191644432637No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG17330.10559005832738767No Hit
CTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGACGATG17300.1054072711519796No Hit
CCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT17100.10418868998259256No Hit
GTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGCGA16670.10156874046841041No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG16450.10022830118208467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)