FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465032
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16940.3642760068124344No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACACCACTCAGGCTGCTGTCATAGGA16730.35976018854616454No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC14630.3146020058834661No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14570.3133117720931033No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14470.3111613824424986No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13700.2946033821328425No Hit
GTCCTGGGCCCAGTCTGTGCTGATGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG13190.28363639491475856No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12260.26363777116413495No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACACCACTCAGG12080.2597670697930465No Hit
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA11680.25116551119062774No Hit
GATTATTACTGTCAGTCCTATGACAGCAGCCTGAGTGGTGTGATATTCGG11410.2453594591339951No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT10810.23245712123036696No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10720.23052177054482273No Hit
GTCATAGGACTGACAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG10160.21847958850143645No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTGACAGCAATCGG10020.2154690429905899No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9820.21116826368938052No Hit
GTGCTGATGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC9530.20493213370262692No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9410.2023516661219013No Hit
GTCCTATGACAGCAGCCTGAGTGGTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG8930.1920297957989988No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8900.19138467890381736No Hit
ATATCACACCACTCAGGCTGCTGTCATAGGACTGACAGTAATAATCAGCC8810.18944932821827315No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8690.18686886063754754No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT8620.18536358788212423No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG8590.18471847098694283No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8420.18106280858091486No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8390.18041769168573346No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8360.17977257479055206No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8330.17912745789537063No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8260.17762218513994738No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8230.17697706824476594No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG8170.17568683445440314No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG8110.17439660066404034No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7470.1606341069001703No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7450.16020402897004937No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGATGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC7350.1580536393194447No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG7280.1565483665640214No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGATGCAGCCGCCCTCAGTGTCT7270.15633332759896093No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7070.15203254829775156No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTG6990.1503122365772678No Hit
GTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC6990.1503122365772678No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGTCAGT6860.1475167300314817No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6700.1440761065905142No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACACCACTCAGGC6540.1406354831495467No Hit
GGATGAGGCTGATTATTACTGTCAGTCCTATGACAGCAGCCTGAGTGGTGTGATATTCGGCGGAGGGACCAAG6400.13762493763870015No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG6310.13568958695315592No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAC6170.13267904144230935No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC6120.131603846617007No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6050.13009857386158372No Hit
GTGTATTACTGTGCGAGACACGTAGGGGGGACAGTTGCTGGGAGCGTAAA6000.1290233790362814No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT5920.12730306731579763No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC5780.12429252180495105No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT5730.12321732697964871No Hit
CAGTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATA5730.12321732697964871No Hit
GTCTGTGCTGATGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG5660.12171205422422543No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG5600.12042182043386261No Hit
CATCTATGGTGACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTC5540.1191315866434998No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC5490.11805639181819746No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGTCAGTCCTAT5440.11698119699289511No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG5390.11590600216759277No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG5330.11461576837722995No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC5280.1135405735519276No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT5180.11139018390132291No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC5160.11096010597120198No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC5150.1107450670061415No Hit
ACTCAGGCTGCTGTCATAGGACTGACAGTAATAATCAGCCTCATCCTCAG5120.11009995011096012No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5120.11009995011096012No Hit
CCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTC5100.10966987218083916No Hit
GGCCCAGTCTGTGCTGATGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC5050.10859467735553682No Hit
CCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTG4990.10730444356517402No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA4940.10622924873987168No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC4910.10558413184469026No Hit
GGGTCCTGGGCCCAGTCTGTGCTGATGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC4870.10472397598444837No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT4840.10407885908926698No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG4820.10364878115914603No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4790.10300366426396464No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT4780.10278862529890416No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG4730.10171343047360182No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTGACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGC4700.1010683135784204No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAG4660.10020815771817854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)