FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465032
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16060.34535257788711315No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACACCACTCAGGCTGCTGTCATAGGA15780.33933148686542No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15580.3350307075642106No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC15330.3296547334376989No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15300.3290096165425175No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13210.2840664728448795No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12900.27740026492800496No Hit
GTCCTGGGCCCAGTCTGTGCTGATGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG11310.2432090694833904No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10810.23245712123036696No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACACCACTCAGG10740.23095184847494368No Hit
GATTATTACTGTCAGTCCTATGACAGCAGCCTGAGTGGTGTGATATTCGG10430.2242856405580691No Hit
GTCATAGGACTGACAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG10270.2208450171171016No Hit
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA10020.2154690429905899No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9880.21245849747974335No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9770.21009306886407816No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9530.20493213370262692No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTGACAGCAATCGG9490.20407197784238504No Hit
GTCCTATGACAGCAGCCTGAGTGGTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG9230.19848096475081284No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8950.1924598737291197No Hit
GTGCTGATGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC8950.1924598737291197No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT8830.18987940614839408No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT8830.18987940614839408No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8610.18514854891706378No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8560.18407335409176143No Hit
ATATCACACCACTCAGGCTGCTGTCATAGGACTGACAGTAATAATCAGCC8550.18385831512670095No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8220.17676202927970547No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8100.17418156169897986No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7910.17009582136283094No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7850.16880558757246814No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG7790.1675153537821053No Hit
GTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC7650.16450480827125874No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGATGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC7620.16385969137607734No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7620.16385969137607734No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7550.16235441862065406No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7290.15676340552908186No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTG7290.15676340552908186No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG7230.15547317173871905No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG7150.1537528600182353No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7090.1524626262278725No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGTCAGT6870.14773176899654217No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6780.14579641831099796No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC6650.14300091176521187No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGATGCAGCCGCCCTCAGTGTCT6300.13547454798809544No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6230.13396927523267216No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACACCACTCAGGC6080.13074369075676512No Hit
GGATGAGGCTGATTATTACTGTCAGTCCTATGACAGCAGCCTGAGTGGTGTGATATTCGGCGGAGGGACCAAG5980.12859330110616043No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAC5970.12837826214109999No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5900.1268729893856767No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGTCAGTCCTAT5720.12300228801458825No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC5630.12106693732904401No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG5600.12042182043386261No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC5590.12020678146880215No Hit
CATCTATGGTGACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTC5540.1191315866434998No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5500.11827143078325791No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG5470.11762631388807653No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC5380.11569096320253229No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5370.11547592423747184No Hit
GTCTGTGCTGATGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG5350.11504584630735089No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC5320.1144007294121695No Hit
GTGTATTACTGTGCGAGACACGTAGGGGGGACAGTTGCTGGGAGCGTAAA5210.11203530079650433No Hit
CAGTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATA5160.11096010597120198No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT5070.10902475528565778No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC5060.1088097163205973No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA5060.1088097163205973No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT4970.10687436563505308No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT4950.10644428770493213No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT4840.10407885908926698No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC4830.1038638201242065No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT4810.10343374219408558No Hit
GGGTCCTGGGCCCAGTCTGTGCTGATGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC4800.1032187032290251No Hit
GCTTGGTCCCTCCGCCGAATATCACACCACTCAGGCTGCTGTCATAGGACTGACAGTAATAATCAGCCTCATCCT4780.10278862529890416No Hit
GTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTACATCATAACCT4770.10257358633384368No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT4770.10257358633384368No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGC4750.10214350840372274No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT4720.10149839150854134No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG4690.10085327461335995No Hit
GCCCCCAAACTCCTCATCTATGGTGACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGT4670.100423196683239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACC158.20285E-489.9906169
ATCGGGA200.007764369251.0480843
AGTACGT200.00776767251.04259513
TCTGGAG3400.046.43537570
CTATACC306.7465845E-445.3760764
CCTATAC306.7465845E-445.3760763
CCGAAAC357.0381927E-444.9865168
GTATCAA10800.040.334291
GGTATCA4000.039.1368681
TATGCCA350.001440508138.893785
CCATCGG350.001440508138.893781
TACGTAC350.0014412738.88959512
TAAGTCA800.001700969837.5905470
ACCAGAA850.002290570235.37933370
GTGGTAT1650.035.063331
TTAACGG400.002683446734.2642735
CCCCTAT400.002774438634.032061
CCCTATA400.002775902434.0283972
GAATACG400.002775902434.0283979
ATACGTA400.002775902434.02839711
Produced by FastQC (version 0.11.5)