FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979449
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39850.4068614088125058No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35970.3672472992468214No Hit
ATATGGTACCTCTCCGTGGACGTTCGGCCGAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT32470.3315129220612814No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25360.2589210872643701No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25290.2582063997206593No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24760.25279519403256323No Hit
GTACCATATTGTTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGCGTGAAGTCTGTC24240.24748608656499724No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21820.22277831719670957No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG21260.21706081684702316No Hit
GTCTGGGACAGACTTCACGCTCACCATCAGCAGACTGGAGCCTGAAGATT21110.21552934353907147No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20510.2094034503072646No Hit
GTATTTCTGTCAACAATATGGTACCTCTCCGTGGACGTTCGGCCGAGGGA20090.2051153250449998No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20050.20470693216287933No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCTCCCTCTCCTGCAGGGCCAGTCAGA20000.20419644106022877No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19500.19909153003372307No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19270.19674327096153044No Hit
GTGTATTTCTGTCAACAATATGGTACCTCTCCGTGGACGTTCGGCCGAGGGACCAAGGTGGAAATCGAACGAA19180.1958243869767594No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19180.1958243869767594No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGGCAGGTTTCTGTTGGTACCAGGCTAAGTAGTTGCCGCCAATAGTCTGA18880.19276144036085596No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18810.19204675281714517No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18590.18980059196548263No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18380.18765652933435023No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17750.181224341440953No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT17450.17816139482504958No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCTCCCTCTCCTGCAGGGCCAGTCAGACTATTGGCGG16950.17305648379854388No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCC16220.16560331369984552No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCTCCCTCTCCTGC15910.16243826886341198No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15800.16131518843758072No Hit
CTACTTAGCCTGGTACCAACAGAAACCTGCCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCA15090.15406621477994262No Hit
GAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGCGTGAAGTCTGTCCCAGACCCACTGCCA14670.1497780895176778No Hit
CTCCTGCAGGGCCAGTCAGACTATTGGCGGCAACTACTTAGCCTGGTACC14650.14957389307661756No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14460.1476340268865454No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13960.14252911586003966No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13120.13395286533551007No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12710.1297668382937754No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGCGAAATTGTGTTGACGCAGTCTC12580.1284395614268839No Hit
ACAGAAACCTGCCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT12310.1256829094725708No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTCGGCCGAACGTC12190.12445773082620942No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12100.12353884684143839No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12090.12343674862090828No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11220.11455420343478834No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11150.11383951589107753No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11080.11312482834736673No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11000.11230804258312581No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10930.11159335503941502No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10840.11067447105464398No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10720.10944929240828262No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10680.10904089952616215No Hit
GTACCTCTCCGTGGACGTTCGGCCGAGGGACCAAGGTGGAAATCGAACGA10650.10873460486457182No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10380.10597795291025873No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT10320.10536536358707804No Hit
GGGACAGACTTCACGCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGC10260.10475277426389734No Hit
GAGAGGTACCATATTGTTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGCGTGAAGT10260.10475277426389734No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10250.10465067604336724No Hit
CAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGCGTGAAGTCTGTCCCAGACCCACTGCCA10200.10414018494071667No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10180.10393598849965645No Hit
GACTTCACGCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTTCTGTCAACAATATGGTACCTCT10090.10301710451488541No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10090.10301710451488541No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10070.10281290807382518No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9990.10199612230958426No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9970.10179192586852402No Hit
TCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACG9960.10168982764799392No Hit
CTCCGTGGACGTTCGGCCGAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC9810.1001583543400422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)