FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979449
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49370.5040589147571747No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41430.42299292765626384No Hit
ATATGGTACCTCTCCGTGGACGTTCGGCCGAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT31050.31701497474600515No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26190.2673952395683696No Hit
GTACCATATTGTTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGCGTGAAGTCTGTC25390.2592273819259604No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25240.25769590861800873No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24430.24942595275506946No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23430.239216130702058No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22920.23400912145502217No Hit
GTCTGGGACAGACTTCACGCTCACCATCAGCAGACTGGAGCCTGAAGATT22180.2264538531357937No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19980.2039922446191685No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCTCCCTCTCCTGCAGGGCCAGTCAGA19720.20133769088538558No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19460.1986831371516026No Hit
GTATTTCTGTCAACAATATGGTACCTCTCCGTGGACGTTCGGCCGAGGGA19320.197253762064181No Hit
GTGTATTTCTGTCAACAATATGGTACCTCTCCGTGGACGTTCGGCCGAGGGACCAAGGTGGAAATCGAACGAA18970.193680324345627No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18400.18786072577541046No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG17880.1825516183078445No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17670.18040755567671213No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCTCCCTCTCCTGCAGGGCCAGTCAGACTATTGGCGG17070.17428166244490526No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16970.17326068023960411No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGGCAGGTTTCTGTTGGTACCAGGCTAAGTAGTTGCCGCCAATAGTCTGA16850.17203550159324274No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCTCCCTCTCCTGC16820.1717292069316524No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT16420.16764527811044783No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16090.16427603683295403No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15840.1617235813197012No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14970.15284103613358122No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCC14920.15233054503093066No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14760.15069697350244882No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14460.1476340268865454No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14300.14600045535806358No Hit
GAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGCGTGAAGTCTGTCCCAGACCCACTGCCA13880.14171233009579876No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13660.13946616924413624No Hit
CTACTTAGCCTGGTACCAACAGAAACCTGCCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCA13590.13875148170042545No Hit
CTCCTGCAGGGCCAGTCAGACTATTGGCGGCAACTACTTAGCCTGGTACC13250.13528014220240156No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGCGAAATTGTGTTGACGCAGTCTC12960.13231929380702825No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12380.12639759701628162No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12370.12629549879575147No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12270.12527451659045036No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTCGGCCGAACGTC11940.12190527531295656No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11680.1192507215791736No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11560.11802554293281221No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11560.11802554293281221No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGCGAAATTGTGTTGACGCAGTC11360.11598357852220993No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11210.11445210521425822No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11110.11343112300895707No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10830.11057237283411388No Hit
ACAGAAACCTGCCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT10750.10975558706987296No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10740.10965348884934285No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10570.1079178191003309No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10500.10720313155662009No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10340.10556956002813828No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10310.10526326536654794No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10300.10516116714601781No Hit
CTCCGTGGACGTTCGGCCGAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC10170.10383389027912632No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG10070.10281290807382518No Hit
GTACCTCTCCGTGGACGTTCGGCCGAGGGACCAAGGTGGAAATCGAACGA9900.10107723832481323No Hit
GAGAGGTACCATATTGTTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGCGTGAAGT9870.1007709436632229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10700.044.4797671
GTATCAA25800.034.9176031
TTAACGG807.453855E-834.30544335
TAACGGC851.2604141E-732.3141336
TACGTAT950.00491467230.28125270
ATCAACG30250.029.5486553
CGGTACC953.0262163E-729.24153945
TATCAAC30350.029.227332
CAACGCA30650.029.052145
TCAACGC30700.029.0048264
AACGCAG30950.028.9901586
GAATACG2000.028.8885829
ATACGTA2000.028.88858211
AATACGT2000.028.88858210
TACGTAC2100.027.51012412
AACGGCC1005.1973257E-727.48828337
AACGTCA500.00802902827.38928833
ATAATAC756.824994E-527.1892553
ACGTACC2250.027.18925513
ACGCAGA33150.027.0662257
Produced by FastQC (version 0.11.5)