FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004689707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323718
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG25350.7830889848571905No Hit
GTATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGC24330.7515800789576113No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT20780.6419167299933892No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA19260.5949622819861732No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT18400.5683959495610377No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT17130.52916427260764No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT12330.3808870683743258No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC12160.37563558405772923No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC11630.35926330942363416No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC10770.33269697699849865No Hit
GGTATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCG10630.3283722252083604No Hit
GAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT9990.3086019313105851No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT9290.2869781723598935No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC9290.2869781723598935No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG9210.28450688562267157No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC8290.2560870881446197No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCT8280.255778177302467No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC8080.2495999604594122No Hit
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCC7880.24342174361635746No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA7670.23693461593114995No Hit
GTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC7530.23260986414101165No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT7180.2217979846656658No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC6600.203881155820807No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT6470.19986531487282141No Hit
GTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT6160.19028907876608653No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT5920.18287521855442082No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCA5800.17916828844858795No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT5710.17638809086921334No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCA5700.17607918002706058No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC5670.17515244750060238No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT5670.17515244750060238No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT5620.1736078932898387No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT5560.17175442823692225No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG5260.16248710297234012No Hit
CCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGT5110.15785344034004906No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT5050.15599997528713264No Hit
TATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCC4970.15352868854991072No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA4910.1516752234969943No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC4870.15043958012838335No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG4780.1476593825490087No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA4730.14611482833824502No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC4530.13993661149519027No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT4490.1387009681265793No Hit
ACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG4490.1387009681265793No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC4380.13530294886289917No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA4370.13499403802074644No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG4320.13344948380998278No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT4270.13190492959921907No Hit
CCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA4180.12912473201984442No Hit
GGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGT4130.12758017780908076No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4040.1247999802297061No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA3990.1232554260189424No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGG3920.12109305012387324No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG3810.11769503086019313No Hit
GTATCAACGCAGAGTACGGGTGGCGGCGACGACCCATTCGAACGTCTGCC3790.11707720917588765No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC3720.11491483328081849No Hit
ATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCC3720.11491483328081849No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3660.11306136822790205No Hit
CCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGA3630.11213463570144384No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3630.11213463570144384No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA3620.11182572485929111No Hit
TGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA3580.11059008149068017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.11059008149068017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.10873661643776374No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCG3460.1068831513848473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.1068831513848473No Hit
GGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT3450.10657424054269457No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCA3390.10472077548977814No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3390.10472077548977814No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT3340.10317622127901445No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA3290.10163166706825075No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC3260.10070493454179255No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACCCCGTA3240.10008711285748707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)