FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511463
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG46880.31016306717398967No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45230.29924649164418843No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA34610.22898344187055852No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG31790.21032602187417093No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC28610.18928680358037214No Hit
CTCCTGTGCAGCCTCTAAATTCACTTTCTTTAACGCCTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG27480.1818106033690537No Hit
GTAATATACGGCTGTGTCTTCAGTCTTCAGGCTGTTCATTTGCAGATACA24240.16037441869235303No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC22510.14892855465201596No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCCAACCCTTTTCCCC22230.1470760448651406No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21370.14138619337688055No Hit
CGTCCATACCGTGCCTCGGGTCCCCAAACCATAGATTGCGGTCTGTGGTA20810.1376811738031298No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19070.12616914869897577No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18850.12471360529500226No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC18800.12438279997591738No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC18180.12028081401926477No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT18030.11928839806201012No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17610.11650963338169706No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT17570.11624498912642917No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA17230.11399551295665195No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC16640.11009201019145026No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG16580.10969504380854841No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT16490.10909959423419562No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC16440.10876878891511073No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA16360.1082395004045749No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT15730.10407135338410534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG306.8067457E-445.3009832
GTGGTAT3850.043.2375561
GTACGGA651.3047611E-841.812141
TACGTAT901.0020351E-438.3280770
GTATCAA29250.035.308031
CTCGATA400.002802676833.9667412
ATCAACG33500.030.6248173
TATCAAC33750.030.4019952
TCGATAT450.004985903430.19665713
TCAACGC34000.030.1754534
CAACGCA34450.029.7822745
AACGCAG34700.029.665616
CGCAGAG35900.028.4847358
ACGCAGA36550.028.071127
AGAGTAC36850.027.65453711
GCAGAGT38100.026.8399479
GGTATCA18700.026.7055471
GCGTAGA9850.026.61563570
CAGAGTA38650.026.45800810
CCGTTAA1209.8394594E-825.49361616
Produced by FastQC (version 0.11.5)