FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056428
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT52290.49496984176867714No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48370.4578636688917749No Hit
CTCCTAACCTGCTCATTTACTGGGCATCTGCCCGGGAGTCCGGGGTCCCT47360.4483031498597159No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42230.3997432858651986No Hit
GGTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCTTACTGGTGGAGCTGAATAAAAGACT40730.38554449522352685No Hit
CTATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC39820.376930562234246No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31910.302055606250497No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30470.2884247672344921No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29850.2825559337692678No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25690.2431779543896981No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25050.23711980371591818No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23710.22443555074269142No Hit
CTTTTATTCAGCTCCACCAGTAAGAACTACTTAGCTTGGTACCAGCAAAA22830.21610559356624398No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22440.21241390799940932No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22390.2119406149780203No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22380.21184595637374243No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20980.19859375177484884No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC20340.1925356011010689No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20080.1900744773898458No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATT19820.1876133536786227No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19190.18164986160912056No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17760.16811368119739348No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17670.16726175375889318No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATTCAGCTCCAC17210.16290745796211384No Hit
TTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTT16890.15987838262522386No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16510.15628135566266702No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16490.1560920384541114No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16260.15391489055572172No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15960.15107513242738738No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15660.14823537429905304No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15660.14823537429905304No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC15550.14719412965199713No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15530.1470048124434415No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15440.1461528850049412No Hit
GAACTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAACCTGC14840.1404733687482725No Hit
GCCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGC14790.14000007572688342No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCC14710.13924280689266091No Hit
GGACTATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT14700.13914814828838312No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT14580.1380122450370494No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATAGTCCTCCGTGGACGTT14520.1374442934113825No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATAGTCCT14490.13716031759854908No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14190.13432055947021473No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATTCAGCTCCACCAGTAAGAACTA14110.13356329063599223No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14070.13318465621888098No Hit
GTCAGGGACCCCGGACTCCCGGGCAGATGCCCAGTAAATGAGCAGGTTAGGAGGCTGTCCTGGTTTTTGCTGGTA13980.1323327287803807No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13840.13100750832049132No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13330.1261799195023229No Hit
AACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTGCCCGGGAG13160.12457072322960012No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13060.12362413718682201No Hit
GAATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA12960.12267755114404388No Hit
GTTTATTACTGTCAGCAATATTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAA12870.12182562370554359No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12810.12125767207987671No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12710.12031108603709859No Hit
GCTCATTTACTGGGCATCTGCCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT12510.11841791395154236No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12260.11605144884459709No Hit
CTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTGCCCGGGAGTC11760.11131851863070649No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11740.11112920142215088No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11720.11093988421359526No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11660.11037193258792838No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11630.11008795677509495No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11430.10819478468953872No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11350.10743751585531622No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11210.10611229539542685No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11180.10582831958259342No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11110.10516570935264874No Hit
TCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCT11100.10507105074837092No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11050.10459775772698188No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11000.1041244647055928No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10830.10251526843287001No Hit
GTAAATGAGCAGGTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTA10800.10223129262003658No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10750.10175799959864752No Hit
GCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGG10690.10119004797298065No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10650.10081141355586941No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10610.10043277913875814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT450.00995877247.93218670
CGCGTAT1000.044.6535939
ATCTCGT4400.043.4843637
CGTATGC4450.043.48340241
ATGCCGT4350.043.21971544
GGTATCA9550.041.358291
CTCTTAT3750.041.0592081
TCGTATG4550.040.13369840
GTATGCC5200.039.97526642
CTTATAC13000.039.9091341
TATACAC13550.039.2568663
GCCGTCT3850.039.08742546
CGAGACT13150.038.96142223
TTATACA13400.038.9473232
ATCGCGT1500.038.72178337
CCGTCTT4000.038.59977347
ACGAGAC13500.038.44011722
TACACAT14000.037.995045
CACGAGA13700.037.8771621
TGCCGTC4450.037.64692745
Produced by FastQC (version 0.11.5)