FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056428
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117181.109209524927397No Hit
ATATTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT52560.49752562408417794No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48630.4603247926029981No Hit
CTATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC46040.43580821409504483No Hit
CTCCTAACCTGCTCATTTACTGGGCATCTGCCCGGGAGTCCGGGGTCCCT41380.3916973045015846No Hit
GGTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCTTACTGGTGGAGCTGAATAAAAGACT37460.3545911316246824No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33480.3169170071221134No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31870.3016769718333857No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30990.2933470146569383No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28130.26627465383348414No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25350.23995956184425252No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23230.21989193773735644No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21430.20285338896735036No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20770.19660592108501476No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19890.18827596390856735No Hit
CTTTTATTCAGCTCCACCAGTAAGAACTACTTAGCTTGGTACCAGCAAAA19790.18732937786578924No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19680.18628813321873333No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18930.17918873789789744No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18740.17739022441661903No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18350.17369853884978437No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18240.17265729420272843No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17940.1698175360743941No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17830.16877629142733816No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17740.16792436398883787No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATT17390.16461131283911445No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17320.16394870260916977No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17120.16205553052361352No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16500.15618669705838922No Hit
TTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTT16360.15486147659849986No Hit
GGACTATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT16260.15391489055572172No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15900.1505071808017205No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15470.14643686081777463No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATTCAGCTCCAC15220.14407039571082933No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14750.1396214413097722No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14590.1381069036413272No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14420.1364977073686044No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATAGTCCTCCGTGGACGTT14080.1332793148231588No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATAGTCCT14000.1325220459889363No Hit
GTAATAAACGGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA13960.13214341157182505No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT13800.13062887390338007No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGACTCCCGGGC13790.13053421529910225No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13600.12873570181782384No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG13580.1285463846092682No Hit
GTCAGGGACCCCGGACTCCCGGGCAGATGCCCAGTAAATGAGCAGGTTAGGAGGCTGTCCTGGTTTTTGCTGGTA13450.12731582275365666No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13220.125138674855267No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCC13120.12419208881248889No Hit
GTTTATTACTGTCAGCAATATTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAA13050.12352947858254419No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12920.12229891672693265No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12870.12182562370554359No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC12860.12173096510126578No Hit
GCCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGC12840.12154164789271016No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12490.11822859674298675No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12470.11803927953443112No Hit
GAACTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAACCTGC12310.11652474186598615No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12230.11576747303176364No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC12200.11548349721893021No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12120.11472622838470771No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATTCAGCTCCACCAGTAAGAACTA11990.11349566652909615No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11750.11122386002642869No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11600.10980398096226152No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11500.10885739491948339No Hit
GGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATAGTCCTCCGT11320.10715354004248279No Hit
AACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTGCCCGGGAG11240.10639627120826028No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11210.10611229539542685No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11200.10601763679114905No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11020.10431378191414845No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10990.10402980610131499No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10960.10374583028848156No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10890.10308322005853689No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG10780.10204197541148095No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10760.10185265820292531No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10690.10119004797298065No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10670.10100073076442502No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10630.10062209634731378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)