FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004689713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633790
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT39150.617712491519273No Hit
GTAATACACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCACATACA23680.37362533331229586No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17580.2773789425519494No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17480.2758011328673535No Hit
CTATTATATTGTTGGCAGTAATAAGTTGCAACATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC15540.24519162498619415No Hit
ATATAATAGTTATTCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT13290.20969090708278768No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12890.20337966834440432No Hit
GGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGCCTTTTT12460.19659508670064216No Hit
ATTAGTAGGTGGTTGGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAA11800.18618154278230958No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11570.18255258050773915No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACTTGGTTAACATCACTTGCCG11210.17687246564319412No Hit
GTGTATTACTGTGCGAAAGGGGACTCCCCCTTAAGACCAGTGGCCCTTGGGGGCCAGGGAACCCTGGTCACCGT11120.17545243692705786No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11110.17529465595859828No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11030.1740324082109216No Hit
GACTATTAGTAGGTGGTTGGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT11000.17355906530554285No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10880.17166569368402784No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10810.17056122690481074No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10800.17040344593635115No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10520.1659855788194828No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATGTTGCAACTTA10380.16377664526104863No Hit
CTCCTGTGCAGCCTCTGGATTCAAATTCAGTAGCTATGTCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGA10280.16219883557645276No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10280.16219883557645276No Hit
GTCTGCATCTGTAGGAGACTTGGTTAACATCACTTGCCGGGCCAGTCAGA10240.1615677117026144No Hit
CAGTAATACACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCACATA10150.16014768298647816No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9920.15651872071190773No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9600.15146972972120104No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9530.15036526294198393No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9310.14689408163587309No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9230.1456318338881964No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9150.14436958614051973No Hit
CCTACTAATAGTCTGACTGGCCCGGCAAGTGATGTTAACCAAGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA8660.1366383186860001No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8370.13206267060067214No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8030.12669811767304628No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT7670.12101800280850124No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7620.1202290979662033No Hit
CTAATAGTCTGACTGGCCCGGCAAGTGATGTTAACCAAGTCTCCTACAGA7420.11707347859701163No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7270.11470676407011786No Hit
CAATATAATAGTTATTCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT7220.11391785922781994No Hit
GGACTGGAGTGGGTCTCAGGTATTAGTGGAAGCGGTGATAGGACATACTA7180.1132867353539816No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7160.11297117341706243No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7130.11249783051168367No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7080.11170892566938576No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7070.11155114470092617No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7060.11139336373246658No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG6920.10918443017403241No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCCAACCACCTACTAATAGTCTGA6800.1072910585525174No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC6780.10697549661559823No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6750.10650215371021947No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6670.10523990596254279No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6540.10318875337256819No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6470.10208428659335111No Hit
ACTTTAGAAGGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC6350.1001909149718361No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6350.1001909149718361No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6350.1001909149718361No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)