FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633790
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67661.067546032597548No Hit
GTAATACACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCACATACA20600.32502879502674387No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18390.2901592009971757No Hit
CTATTATATTGTTGGCAGTAATAAGTTGCAACATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC16780.26475646507518263No Hit
ATATAATAGTTATTCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT14580.23004465201407406No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13620.21489767904195398No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13090.20653528771359597No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12740.20101295381751055No Hit
GGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGCCTTTTT12230.19296612442607172No Hit
GTGTATTACTGTGCGAAAGGGGACTCCCCCTTAAGACCAGTGGCCCTTGGGGGCCAGGGAACCCTGGTCACCGT11630.18349926631849667No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10610.16740560753561906No Hit
CTCCTGTGCAGCCTCTGGATTCAAATTCAGTAGCTATGTCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGA10580.1669322646302403No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10530.1661433597879424No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10170.16046324492339734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10090.15920099717572067No Hit
ATTAGTAGGTGGTTGGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAA9890.15604537780652897No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9830.15509869199577148No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9830.15509869199577148No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9700.15304753940579688No Hit
GACTATTAGTAGGTGGTTGGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT9650.15225863456349895No Hit
GTCTGCATCTGTAGGAGACTTGGTTAACATCACTTGCCGGGCCAGTCAGA9520.15020748197352435No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACTTGGTTAACATCACTTGCCG9330.14720964357279226No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATGTTGCAACTTA8960.14137174773978764No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8810.13900503321289387No Hit
CAGTAATACACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCACATA8720.1375850044967576No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8690.13711166159137883No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8670.13679609965445969No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTCGATGGGACCCCA8520.13442938512756591No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8280.1306426418845359No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8040.12685589864150587No Hit
CCTACTAATAGTCTGACTGGCCCGGCAAGTGATGTTAACCAAGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA7610.12007131699774372No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7610.12007131699774372No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7460.11770460247084998No Hit
CAATATAATAGTTATTCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT7380.1164423547231733No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT7250.1143912021331987No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7250.1143912021331987No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7170.11312895438552201No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7160.11297117341706243No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7130.11249783051168367No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7030.11092002082708782No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6880.10855330630019408No Hit
ATTATATTGTTGGCAGTAATAAGTTGCAACATCATCAGGCTGCAGGCTGC6860.10823774436327491No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6750.10650215371021947No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6670.10523990596254279No Hit
GGACTGGAGTGGGTCTCAGGTATTAGTGGAAGCGGTGATAGGACATACTA6640.10476656305716404No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6510.10271541046718943No Hit
GTATTACTGTGCGAAAGGGGACTCCCCCTTAAGACCAGTGGCCCTTGGGGGCCAGGGAACCCTGGTCACCGTCTC6500.10255762949872985No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6480.1022420675618107No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC6470.10208428659335111No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6440.10161094368797237No Hit
CTAATAGTCTGACTGGCCCGGCAAGTGATGTTAACCAAGTCTCCTACAGA6350.1001909149718361No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)