FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127795
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55610.4930860661733737No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39420.3495316081380038No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38790.34394548654675716No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36280.3216896687784571No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA30500.27043921989368636No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30340.26902052234670304No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT28430.25208482037959024No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28130.24942476247899661No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27700.24561201282147907No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27540.2441933152744958No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT26790.23754317052301172No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGAGTGGAAGGAGA26050.23098169436821409No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26030.23080435717484116No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24640.218479422235424No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24370.21608537012488974No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23900.21191794608062636No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23600.20925788818003271No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23110.20491312694239644No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21530.19090348866593665No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA21370.18948479111895336No Hit
GTAATAGGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATACACTGCCG21240.18833209936202944No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21130.18735674479847844No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21020.18638139023492745No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20820.18460801830119836No Hit
GAGTGAATTCTGTCCCAGATACACTGCCGCTGAACCTTGATGGGACCCCA20500.1817706232072318No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20400.18088393724036725No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19270.1708643858147979No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19000.16847033370426362No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT18380.16297288070970345No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17860.1583621136820078No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17450.15472670121786317No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTA17270.153130666477507No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA16770.14869723664318427No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16310.14461848119560736No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16020.14204709189170017No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACCTATTACTGCCAACAATATAATAATTAT15610.13841167942755553No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGA15500.13743632486400453No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15440.1369043132838858No Hit
TTATTATATTGTTGGCAGTAATAGGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC15310.1357516215269619No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15100.13388958099654635No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15010.13309156362636826No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14920.13229354625619016No Hit
GGCCAAAAGTGTACGGATAATTATTATATTGTTGGCAGTAATAGGTTGCA14770.13096351730589337No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAA14770.13096351730589337No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCTTCCACT14720.1305201743224611No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14630.129722156952283No Hit
ACCTTGATGGGACCCCACTTTCTAAAATAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG14580.12927881396885074No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14520.128746802388732No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14480.12839212800198618No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14450.12812612221192682No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14440.12803745361524035No Hit
TTCTAAAATAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT14150.12546606431133317No Hit
ACCTATTACTGCCAACAATATAATAATTATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13530.11996861131677299No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13460.1193479311399678No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13400.11881591955984908No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13280.11775189639961162No Hit
CAGATACACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAAATAGACGCCTTATAGATCAGGAGTTTAGGGG13280.11775189639961162No Hit
CAGTAATAGGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATACACTGCCG13210.11713121622280644No Hit
CCTTCCACTCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12790.11340713516197537No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12730.11287512358185664No Hit
GTAGTACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG12680.11243178059842436No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12550.11127908884150045No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12430.11021506568126299No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12320.109239711117712No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11900.1055156300568809No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGATAATT11880.10533829286350799No Hit
ATGTACTACTGTGCGAGACGTCTCGAATGTAGTGGTGGAAGCTGCCCAAC11880.10533829286350799No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11630.10312157794634663No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11590.1027669035596008No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11570.1025895663662279No Hit
GTCCCAGATACACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAAAT11550.10241222917285499No Hit
GTCTCCTACAGATGCAGACAGAGTGGAAGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGGGAGCCAGAG11320.10037285144906655No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11290.10010684565900718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGT150.001829838873.53570664
CGTATGG200.002455257368.2806569
GCGTATG200.0040769760.07890768
ACCGGGG700.001001020341.88260770
GTGGTAT2750.040.453451
TGCGTAT400.001636123437.90303867
AATGGTA3900.037.58695270
GTATCAA19550.035.8666571
GGGCGTA553.7289612E-430.647913
GTGTTAA553.729931E-430.64655311
AACGCAG23600.029.4324136
ATCAACG23650.029.3649753
TATCAAC23850.029.261382
TCAACGC23900.029.061684
CAACGCA24200.028.702685
GACAGGT1650.028.6034471
ACGCAGA25300.027.5880157
CGCAGAG25450.027.4254158
GTCTGAG6700.027.34871770
ATACCCT500.00865122626.9737514
Produced by FastQC (version 0.11.5)