FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621347
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT27110.4363101455386442No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATCTATGAGGCGT25970.41796290961411253No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25330.40766270698981405No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23470.37772774311294655No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17490.28148522484215743No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTA16250.26152858225757913No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15150.24382510899706605No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14720.23690466035886548No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13740.22113247509040843No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG13710.22064965309239443No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13550.2180746024363198No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13540.21791366177031515No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13210.21260261979216122No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA13140.21147603513012855No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12960.20857910314204461No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTC12880.2072915778140073No Hit
CTATAAAACTCATTTTTCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCAT12510.2013367731718347No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12400.19956642584578344No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAGCACTATAAAACTCATTTTTCATTCA12380.1992445445137741No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12260.19731325652171816No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11740.18894434188947562No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGG11700.18830057922545695No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCC11650.18749587589543365No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11220.18057542725723308No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATCTATGAGGCGTCTA10900.17542532594508384No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA10730.17268933462300454No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA10610.17075804663094857No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10120.16287195399672003No Hit
GTTTTATAGTGCTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC9870.15884843734660342No Hit
ACTTTAGAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGC9570.1540202173664635No Hit
ACTTATTACTGCCAGCACTATAAAACTCATTTTTCATTCACTTTCGGCCC9540.15353739536844951No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCTTCCACC9480.15257175137242152No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT9330.15015764138235158No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT9120.14677788739625364No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8940.14388095540816967No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8790.1414668454180997No Hit
GTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGAAAAATGAGTTTTATAGTG8790.1414668454180997No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8680.1396964980920484No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8310.13374169344987585No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8260.1329369901198525No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8100.1303619394637779No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7990.12859159213772658No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7970.12826971080571725No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7760.12488995681961931No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7610.12247584682954936No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG7470.12022267750548404No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7290.11732574551740009No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7280.11716480485139544No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7070.1137850508652975No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7070.1137850508652975No Hit
TTCTAAAGTAGACGCCTCATAGATCAGGAACTTAGGGGCTTTCCCTGGTT7060.11362411019929283No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6970.11217564420525085No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6880.11072717821120888No Hit
TCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAGCACTAT6810.10960059354917623No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6790.10927871221716691No Hit
ACCTTGATGGGACCCCGCTTTCTAAAGTAGACGCCTCATAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTG6790.10927871221716691No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTG6780.10911777155116224No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6760.10879589021915291No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATCTATGAGGCGTCTACTTTAGAAAGCGGGGTCCCAT6750.10863494955314826No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6740.10847400888714359No Hit
TCTATGAGGCGTCTACTTTAGAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT6710.10799118688912958No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCA6640.10686460222709693No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6570.10573801756506428No Hit
GTTCCTGATCTATGAGGCGTCTACTTTAGAAAGCGGGGTCCCATCAAGGT6470.10412861090501764No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6440.10364578890700366No Hit
GCCCCTAAGTTCCTGATCTATGAGGCGTCTACTTTAGAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT6400.10300202624298499No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6370.102519204244971No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6260.1007488569189197No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6220.10010509425490105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT150.00224832269.8113647
CGTATGG408.2505903E-751.75768741
CTCTTAT2150.050.4386441
CGTCGTC305.9121643E-446.61047448
CTAACGC306.898597E-445.173713
CTTATAC7950.040.0693781
GTCGTAT1050.039.3119839
ATGTCGT1050.039.17752537
TCGTATG6300.038.83261540
GTAACAA350.001469389838.7390322
CCTAACG350.001472882238.720322
TATACAC8250.038.192323
ATCTCGT5800.037.82657637
TATGGCG557.163895E-637.8061943
TTATACA8350.037.734932
CCCACGA8450.037.69840619
GCCCACG8500.037.47665418
ACGAGAC8250.037.38903422
CGTATGC6650.037.3589341
CACGAGA8300.037.16379521
Produced by FastQC (version 0.11.5)