FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493334
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64390.43118284322194494No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55940.3745980470544433No Hit
CTATAATGCCTATTCCCCAATGTTCACTTTTGGCCAGGGGACCAAACTGG43360.29035701323347624No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42040.28151773146529846No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39910.26725434497573886No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39730.26604898837098734No Hit
TCTATAAGGCGTCTAATTTAGAGACTGGGGTCTCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT38810.2598882768355907No Hit
GAGTATTGGTTCTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCATCATCTATAAGGCGT33160.222053472297557No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30600.20491062280775765No Hit
CCTTATAGATGATGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAAGAACCAATACTCTGA29450.19720973338851186No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29190.19546866273720412No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29170.19533473422556508No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAAGAACCA29140.1951338414581065No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28740.19245527122532535No Hit
GCATTATAGTGTTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC28440.19044634355073947No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAAGTTA27890.1867633094806654No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27580.18468741755026002No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27530.18435259627116238No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27300.1828124183873132No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26170.1752454574797065No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25930.1736383153400378No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25430.17029010254906138No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25380.16995528126996373No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG24500.16406242675784521No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATTTCTTGCCG24200.16205349908325933No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATTTCTTGCCGGGCCAGTCAGA23770.1591740360830196No Hit
GTAGGAGACAGAGTCACCATTTCTTGCCGGGCCAGTCAGAGTATTGGTTCTTGGTTGGCCTGGTATCAGCAGAAA23570.15783475096662905No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21940.14691957726804586No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21750.1456472564074748No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21620.14477672108182094No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21490.14390618575616707No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20940.140223151686093No Hit
CCCCTAACCTCATCATCTATAAGGCGTCTAATTTAGAGACTGGGGTCTCA20250.1356026180345455No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20180.13513386824380882No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGAGACCCCA20140.1348660112205307No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTG19890.13319190482504248No Hit
ACCTTGATGAGACCCCAGTCTCTAAATTAGACGCCTTATAGATGATGAGGTTAGGGGCTTTCCCTGGTTTCTG19810.13265619077848625No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT19470.1303794060806223No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19280.12910708522005124No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19260.12897315670841217No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18710.1252901226383381No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18700.12522315838251857No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18590.12448655156850376No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAGCGAACTGTGGCTGCACCA18200.12187494559154213No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17720.11866066131220476No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17370.11631691235852128No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17130.11470977021885258No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16800.11249994977680813No Hit
GGCCAAAAGTGAACATTGGGGAATAGGCATTATAGTGTTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGG16680.11169637870697378No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16440.11008923656730511No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16270.10895084421837312No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16260.10888387996255358No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16180.10834816591599736No Hit
AATTTAGAGACTGGGGTCTCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC16020.1072767378228849No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15880.10633923824141149No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15510.10386156077608895No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATTTCTTGCCGGGCCAGTCAGAGTATTGGTT15500.1037945965202694No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15320.1025892399155179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC209.761656E-567.1218953
GTGGTAT4500.042.5105361
GTATCAA39150.034.9753951
GACAGGT2850.031.7945841
ATCAACG44650.030.5919533
TCAACGC45100.030.2887424
TATCAAC45700.029.8186342
CAACGCA45850.029.7932855
AACGCAG46150.029.7450686
ACGCAGA48950.028.1121777
CGCAGAG48950.028.0436128
AGAGTAC49650.027.37325111
GCAGAGT50100.027.3329059
AATGGTA5950.026.95649770
TGACACG500.00884742226.850564
CGATTGC550.00890714426.8133763
GCCTGAC7650.023.691677
TAGTCAC1452.1764208E-823.62184538
TGTTAGA22500.023.005670
GAGTACG44000.022.80390212
Produced by FastQC (version 0.11.5)