FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004689725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977906
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75010.37923945829579364No Hit
CACCATAACTGGTCTGCAACTTGAAGATTTTGCAACTTACTATTGTCAACAGAGTTACAATACCCTGTGGACGTT67770.3426350898374341No Hit
GATCTATGGTGCGTCCAATTTGCAAAGTGGAGTCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA66440.33591080668140955No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63640.32175442108977875No Hit
GGGTATTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAAGTTGCAGACCAGTTATGGTGAGAGTGAAAT63050.3187714684115423No Hit
CCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT58340.2949584055056206No Hit
GTTACAATACCCTGTGGACGTTCGGCCAGGGGACCATGCTGCAAATCAAA56280.28454335039177797No Hit
GTATTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAAGTTGCAGACCAGTTATGGTGAGAGTGAAAT55750.2818637488333621No Hit
ACAATACCCTGTGGACGTTCGGCCAGGGGACCATGCTGCAAATCAAACGA52390.26487608612340524No Hit
GTTATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGTGA51720.2614886652854079No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51280.25926409040672305No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50320.25441047248959253No Hit
GTAATAGACAGCCGTGTCCGCGGCGGTAACAGAGCTCAGTTTCAGGGAGAACTGGCTCTTGGACGTGTCTACTGA49210.24879847677291034No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49090.24819177453326902No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT47730.2413158158173341No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG45600.23054685106370068No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39730.20086899984124626No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38990.19712766936345813No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38840.1963692915639065No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG38440.19434695076510208No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38260.1934368974056401No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37690.19055506176734385No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37180.18797657724886824No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC35090.1774098465751153No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG35060.17725817101520497No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34220.17301125533771575No Hit
GTCTCCATCCTCCCTGTCCGCATCTGTGGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGACCATTAGTAC34120.17250567013801466No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34040.17210120197825376No Hit
GTGGAGTCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT34030.17205064345828366No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33950.17164617529852277No Hit
GAGTTACAATACCCTGTGGACGTTCGGCCAGGGGACCATGCTGCAAATCAAACGAACTGTGGCTGCACCAT33570.1697249515396586No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATAACTGGTCTGCAACTTGAAGATTTTGCAACTTA33440.1690676907800472No Hit
GTCTATTACTGTGCGAGGAGGGCAAGGACCCGCCTTCGTTTTGTTTACCAATTTGACCTCTGGGGCCAGGGAACC33360.1686632226202863No Hit
GTCCTGATCTATGGTGCGTCCAATTTGCAAAGTGGAGTCCCATCACGGTT32130.1624445246639628No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30740.15541689038811754No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTGCAGCATGGTCC30220.15278784734967182No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAGTAGGTACTA29840.15086662359080766No Hit
CCATTAGTACCTACTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT29260.14793422943254128No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT28020.14166497295624766No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27950.1413110633164569No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27830.14070436107681558No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26930.1361540942795057No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26890.13595186019962527No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC26810.13554739203986438No Hit
GTCTCTGGTGGGTCCTTCAATGATTACTTCTGGACCTGGATCCGCCAGCC26670.13483957276028286No Hit
GATTTCACTCTCACCATAACTGGTCTGCAACTTGAAGATTTTGCAACTTACTATTGTCAACAGAGTTACAATACC26390.13342393420111975No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26370.13332281716117955No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26250.13271611492153823No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26050.13170494452213605No Hit
GTGGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGACCATTAGTACCTACTTAAATTGGTATCAGCAGAAA25970.13130047636237516No Hit
GTACTAATGGTCTGACTTGCCCGGCAGGTGATGGTGACTCTGTCTCCCACAGATGCGGACAGGGAGGATGGAGA25900.1309465667225844No Hit
GTGCAGCCACAGTTCGTTTGATTTGCAGCATGGTCCCCTGGCCGAACGTC25680.12983427928324198No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25560.12922757704360066No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAGT24710.12493010284614134No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23760.12012704344898088No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGTGATGGGACTCCA23150.11704297373080419No Hit
TTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAAGTTGCAGACCAGTTATGGTGAGAGTGAAATCTGTC22750.11502063293199981No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22540.1139589040126275No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22400.11325108473304597No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22360.11304885065316551No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22270.11259382397343454No Hit
GTCCGCATCTGTGGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGA22080.11163321209400245No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT21840.11041980761471981No Hit
CCATCCTCCCTGTCCGCATCTGTGGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGACCATTAGTACCTA21750.10996478093498882No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21630.10935807869534751No Hit
CCCCTAAGGTCCTGATCTATGGTGCGTCCAATTTGCAAAGTGGAGTCCCA21550.10895361053558662No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21300.1076896475363339No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21130.10683015269684201No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20530.10379664149863543No Hit
GCCAGGGGACCATGCTGCAAATCAAACGAACTGTGGCTGCACCATCTGTC20510.1036955244586952No Hit
TCTATGGTGCGTCCAATTTGCAAAGTGGAGTCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT20390.1030888222190539No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20300.10263379553932289No Hit
CCATAACTGGTCTGCAACTTGAAGATTTTGCAACTTACTATTGTCAACAG20150.10187541773977125No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19840.10030810362069785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)