FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004689726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977906
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108670.5494194365151832No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73760.3729196432995299No Hit
CACCATAACTGGTCTGCAACTTGAAGATTTTGCAACTTACTATTGTCAACAGAGTTACAATACCCTGTGGACGTT66110.3342423755223959No Hit
GGGTATTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAAGTTGCAGACCAGTTATGGTGAGAGTGAAAT64820.3277203264462517No Hit
GATCTATGGTGCGTCCAATTTGCAAAGTGGAGTCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA63900.32306894260900165No Hit
GTATTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAAGTTGCAGACCAGTTATGGTGAGAGTGAAAT56370.284998377071509No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55150.2788302376351556No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51310.2594157659666334No Hit
GTTACAATACCCTGTGGACGTTCGGCCAGGGGACCATGCTGCAAATCAAA51070.25820236148735076No Hit
CCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT49850.25203422205099735No Hit
GTAATAGACAGCCGTGTCCGCGGCGGTAACAGAGCTCAGTTTCAGGGAGAACTGGCTCTTGGACGTGTCTACTGA49700.2512758442514457No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48630.24586608261464396No Hit
ACAATACCCTGTGGACGTTCGGCCAGGGGACCATGCTGCAAATCAAACGA47960.2424786617766466No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG47760.24146749137724444No Hit
GTTATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGTGA47470.24000129429811123No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44790.22645161094612182No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40810.20632931999801812No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37360.18888663060833022No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36470.18438692233099047No Hit
GTGGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGACCATTAGTACCTACTTAAATTGGTATCAGCAGAAA36270.18337575193158825No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36030.1821623474523056No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35910.18155564521266432No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35630.18014000665350122No Hit
GTGGAGTCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT34890.17639867617571311No Hit
GTCTCCATCCTCCCTGTCCGCATCTGTGGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGACCATTAGTAC34700.17543806429628103No Hit
GAGTTACAATACCCTGTGGACGTTCGGCCAGGGGACCATGCTGCAAATCAAACGAACTGTGGCTGCACCAT34650.17518527169643047No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34140.17260678717795486No Hit
GTCCTGATCTATGGTGCGTCCAATTTGCAAAGTGGAGTCCCATCACGGTT33670.1702305367393597No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC33420.16896657374010696No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATAACTGGTCTGCAACTTGAAGATTTTGCAACTTA33170.1677026107408542No Hit
GTCTATTACTGTGCGAGGAGGGCAAGGACCCGCCTTCGTTTTGTTTACCAATTTGACCTCTGGGGCCAGGGAACC33000.16684311590136233No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG32690.16527580178228896No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT31520.1593604549457861No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31030.15688308746725071No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30560.15450683702865556No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29750.15041159691107667No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29650.14990601171137558No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27990.14151329739633733No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27890.14100771219663624No Hit
GTCTCTGGTGGGTCCTTCAATGATTACTTCTGGACCTGGATCCGCCAGCC27810.14060324403687535No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAGTAGGTACTA27720.1401482173571444No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27640.1397437491973835No Hit
GTGCAGCCACAGTTCGTTTGATTTGCAGCATGGTCCCCTGGCCGAACGTC27210.13756973283866877No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT27170.13736749875878834No Hit
GTACTAATGGTCTGACTTGCCCGGCAGGTGATGGTGACTCTGTCTCCCACAGATGCGGACAGGGAGGATGGAGA26680.13489013128025296No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC26540.13418231200067143No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTGCAGCATGGTCC26310.1330194660413589No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26230.132614997881598No Hit
GATTTCACTCTCACCATAACTGGTCTGCAACTTGAAGATTTTGCAACTTACTATTGTCAACAGAGTTACAATACC26190.13241276380171757No Hit
CCATTAGTACCTACTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT25340.12811528960425825No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24930.12604239028548375No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24000.12134044792826353No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23780.12022816048892111No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23550.11906531452960858No Hit
GTCCGCATCTGTGGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGA23360.11810470265017649No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23250.11754855893050528No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGTGATGGGACTCCA23200.11729576633065474No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22890.11572845221158135No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22520.11385778697268728No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAGT22450.1135038773328965No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21940.1109253928144209No Hit
TTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAAGTTGCAGACCAGTTATGGTGAGAGTGAAATCTGTC21830.1103692490947497No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21620.10930752017537738No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21360.10799299865615454No Hit
CCATCCTCCCTGTCCGCATCTGTGGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGACCATTAGTACCTA20960.10597065785735015No Hit
CCCCTAAGGTCCTGATCTATGGTGCGTCCAATTTGCAAAGTGGAGTCCCA20810.1052122800577985No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20470.10349329037881477No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19920.10071257178045873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)