FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449981
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18640.41423971234341006No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17890.39757234194332647No Hit
CATCTACACTGCATCCACCAGGGCCGCTGGTATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCA17130.3806827399379085No Hit
GTGTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC13070.2904567081721228No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12500.2777895066680593No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12250.27223371653469813No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA11950.2655667683746647No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11920.26490007355866135No Hit
GTATGATAATTGGCCCACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCAT11710.260233209846638No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10820.24045459697187213No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10760.23912120733986544No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10520.23378764881183872No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10270.22823185867847756No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9250.2055642349343639No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9160.20356415048635385No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9140.20311968727568497No Hit
GAATTCACTCTCATCATCAGCGACCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAGCAGTATGATAATTGG9060.20134183443300938No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT9000.20000844480100272No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8920.19823059195832712No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG8240.1831188427955847No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8200.1822299163742469No Hit
TTATCATACTGCTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGTCGCTGATGATGAGAGTGAATTCTGTC7280.16178460868347774No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6940.15422873410210652No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC6820.15156195483809315No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6810.1513397232327587No Hit
CTCTTGCAGGGCCAGTCAGAGCATTAGGACCGGCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT6780.1506730284167554No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6730.14956187039008312No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGCATTAGGACCGGCTTAGCCTGGTA6620.14711732273140424No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCTTTCCACCTTGGTCC6610.14689509112606977No Hit
GGCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACACTGCATCCACCAGGGCCG6250.13889475333402965No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6250.13889475333402965No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6200.13778359530735743No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6200.13778359530735743No Hit
GCATCCACCAGGGCCGCTGGTATTCCAGCCAGGTTCAGTGGCAGTGGGTC6180.13733913209668852No Hit
GTCTGGGACAGAATTCACTCTCATCATCAGCGACCTGCAGTCTGAAGATT6060.13467235283267517No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6030.1340056580166718No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5890.13089441554198955No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5790.12867209948864505No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5730.1273387098566384No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5700.12667201504063505No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5670.1260053202246317No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5660.12578308861929727No Hit
GGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATC5660.12578308861929727No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5650.1255608570139628No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5630.12511639380329392No Hit
GAGTGAATTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGAATACCA5590.12422746738195614No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5560.12356077256595278No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5520.12267184614461499No Hit
GCATTAGGACCGGCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACACTGCATCCA5510.12244961453928055No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGCATTAGGAC5510.12244961453928055No Hit
GTTTATTACTGTCAGCAGTATGATAATTGGCCCACGTGGACGTTCGGCCA5490.12200515132861164No Hit
GTGCAGCCACAGTTCGTTTGCTTTCCACCTTGGTCCCTTGGCCGAACGTCCACGTGGGCCAATTATCATACTG5450.12111622490727386No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT5420.12044953009127052No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5280.11733828761658827No Hit
GTAATAAACTCCAAAATCTTCAGACTGCAGGTCGCTGATGATGAGAGTGAATTCTGTCCCAGACCCACTGCCA5250.11667159280058491No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5240.11644936119525047No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5200.11556043477391267No Hit
CACCAGGGCCGCTGGTATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCACTCTCATCATCAGCGA5150.11444927674724044No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5040.11200472908856152No Hit
CATCATCAGCGACCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAGC5030.11178249748322708No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4930.1095601814298826No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA4840.10756009698187256No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG4830.10733786537653812No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC4780.10622670734986588No Hit
CTGGTATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCACT4750.10556001253386255No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA4750.10556001253386255No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT4680.1040043912965214No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4590.10200430684851139No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC4570.10155984363784248No Hit
TAATTGGCCCACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCATCTGTCTT4560.10133761203250805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTC150.001671147475.2279467
TGTGCGT252.8594458E-454.0008912
TTATAAG200.00626345353.8970458
ATTGTCG200.006316404353.78247557
TTGCCGA305.2894757E-447.67153556
GTGGTAT1350.045.000741
TCTAGAT1101.2110468E-744.97503370
GTATCAA14400.043.125711
CGTATGC2300.040.5625941
TGGTATC1500.040.4871642
TCGTATG2300.040.47047840
GGTATCA4900.039.260851
CGTACTA2250.039.19233329
GACCGTA2350.038.8766326
ATCTCGT2050.038.44772337
GTACTAG2300.038.3703330
ACCGTAC2400.038.0921727
GAGACCG2400.038.02432324
GCTTGAA2450.037.9426556
ACTAGAT2350.037.6760932
Produced by FastQC (version 0.11.5)