FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449981
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20310.4513523904342628No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19060.4235734397674568No Hit
CATCTACACTGCATCCACCAGGGCCGCTGGTATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCA15990.35534833692978146No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14890.33090286034299227No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12710.2824563703800827No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA12340.27423380098270816No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12320.2737893377720393No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10680.23734335449718985No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9880.2195648260704341No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9690.2153424255690796No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9670.2148979623584107No Hit
GTGTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC9620.21378680433173847No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9540.21200895148906287No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9210.2046753085130261No Hit
GTATGATAATTGGCCCACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCAT8990.19978621319566825No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8660.1924525702196315No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8360.18578562205959806No Hit
GAATTCACTCTCATCATCAGCGACCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAGCAGTATGATAATTGG8350.18556339045426362No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG8340.18534115884892918No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8330.18511892724359474No Hit
CTCTTGCAGGGCCAGTCAGAGCATTAGGACCGGCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT7900.1755629682142135No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7540.1675626304221734No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC7470.16600700918483224No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7420.16489585115816No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7250.1611179138674744No Hit
TTATCATACTGCTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGTCGCTGATGATGAGAGTGAATTCTGTC7170.15934006102479883No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGCATTAGGACCGGCTTAGCCTGGTA6890.1531175760754343No Hit
GGCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACACTGCATCCACCAGGGCCG6490.1442283118620564No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6400.14222822741404637No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6240.1386725217286952No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6020.13378342641133736No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6010.13356119480600293No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5880.13067218393665508No Hit
GTCTGGGACAGAATTCACTCTCATCATCAGCGACCTGCAGTCTGAAGATT5830.12956102590998286No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT5780.12844986788331064No Hit
GCATCCACCAGGGCCGCTGGTATTCCAGCCAGGTTCAGTGGCAGTGGGTC5740.12756094146197283No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5690.1264497834353006No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5660.12578308861929727No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCTTTCCACCTTGGTCC5660.12578308861929727No Hit
GCATTAGGACCGGCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACACTGCATCCA5570.12378300417128724No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5550.12333854096061835No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5530.12289407774994944No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGCATTAGGAC5510.12244961453928055No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5500.12222738293394611No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5340.11867167724859493No Hit
CTGGTATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCACT5330.1184494456432605No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5330.1184494456432605No Hit
GAGTGAATTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGAATACCA5140.11422704514190599No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5080.11289365550989931No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5070.11267142390456485No Hit
GTAATAAACTCCAAAATCTTCAGACTGCAGGTCGCTGATGATGAGAGTGAATTCTGTCCCAGACCCACTGCCA5040.11200472908856152No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG5010.11133803427255819No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4990.11089357106188928No Hit
GTGCAGCCACAGTTCGTTTGCTTTCCACCTTGGTCCCTTGGCCGAACGTCCACGTGGGCCAATTATCATACTG4870.10822679179787591No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC4870.10822679179787591No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4770.10600447574453144No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC4670.10378215969118695No Hit
GTTTATTACTGTCAGCAGTATGATAATTGGCCCACGTGGACGTTCGGCCA4660.10355992808585252No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4660.10355992808585252No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC4660.10355992808585252No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA4630.10289323326984917No Hit
GTAATATACGGCCGTGTCTGCGGCAGTCACATAGTTCAGCCTCAGGGAGA4580.10178207524317692No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG4540.10089314882183915No Hit
GGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATC4520.10044868561117025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)