FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623227
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50980.31406574681175214No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47130.2903475607539796No Hit
GTAATACAGGGCCGTGTCCTCAACTCTCAGACTGTTCATTTGTAGGTACA46570.2868976427819399No Hit
GAGTTACACCAACTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTT45710.28159955446773616No Hit
GTATTACTGTGCAAAAGATAGGTGGGCTGTAGCAGCAGTTGGCACGACAG36310.2236902170799278No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34460.21229316663658257No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34020.20958251680140857No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33420.20588617611708035No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30680.18900622032531494No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29900.1842009774356883No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29850.1838929490453276No Hit
CAGTAATACAGGGCCGTGTCCTCAACTCTCAGACTGTTCATTTGTAGGTA29180.17976536861449446No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTTAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT27880.17175663046511672No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTC27690.17058612258174613No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27640.17027809419138543No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26470.16307022985694544No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25950.15986673459719436No Hit
GATCTATGCTGCATCCAATTTACACCCTGGGGTCCCATCAAGATTCAGCGGCACTGCTTCTGGGACAGATTTCA25920.15968191756297795No Hit
CTGTATTACTGTGCAAAAGATAGGTGGGCTGTAGCAGCAGTTGGCACGAC25810.15900425510418445No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25370.15629360526901043No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25170.1550614917075677No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25060.1543838292487742No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24900.15339813839962002No Hit
GTCTCCACCCTCCCTGTCTGCATCTGTAGGAGGCAGCGTCACCATCACTT24680.15204281348203302No Hit
CCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG24670.15198120780396088No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24160.1488393182222819No Hit
GATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACCAA23610.1454510059283144No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC23370.14397246965458313No Hit
CTCCTGATCTATGCTGCATCCAATTTACACCCTGGGGTCCCATCAAGATT23190.14286356744928466No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC23130.14249393338085184No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23020.14181627092205834No Hit
ACTTACTACTGTCAACAGAGTTACACCAACTGGACGTTCGGCCAAGGGAC22280.13725745074472023No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22080.13602533718327753No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCAT21250.1309120659032902No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT21220.13072724886907375No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCC21070.12980316369799172No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20950.12906389556112607No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20810.12820141606811616No Hit
GTCTGAGAGTTGAGGACACGGCCCTGTATTACTGTGCAAAAGATAGGTGG19780.1218560312266861No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19690.12130158012403688No Hit
GTAGGAGGCAGCGTCACCATCACTTGCCGGGCAAGTCAGGCCATTAACAGATATTTAAATTGGTATCAGCAGAAA19640.12099355173367618No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19600.12074712902138765No Hit
GTCTGCATCTGTAGGAGGCAGCGTCACCATCACTTGCCGGGCAAGTCAGG19470.11994625520644986No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18500.11397050443345262No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18480.11384729307730836No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18450.11366247604309193No Hit
CACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACCAACTGGACGTTCGG18090.11144467163249501No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCC17910.11033576942719656No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17410.10725548552358974No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17140.10559213221564205No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17030.10491446975684855No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16980.10460644136648786No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16470.10146455178480891No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16270.10023243822336618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTCG200.007046018352.32295651
TGCCCGA400.00821617150.32634470
GTGGTAT4100.050.0762441
CGTATGC3900.043.4263841
TCGTATG3850.042.1554740
GACAGGT4200.040.736631
ATCTCGT3900.040.6121437
CGAGACG4400.040.45282723
TCTCCGA4700.039.30185711
CATCTCG4400.038.32456236
ATGCCGT4350.038.23224644
ACGAGAC4900.037.71861322
ATCTCCG4900.037.697710
CACGAGA5050.036.59713421
CCCACGA5100.036.2271719
TATACAC5300.036.1423153
GCCGTCT4150.035.97101646
CCGTCTT4200.035.57504347
CTACATC4650.035.4690633
GTATGCC4800.035.31911542
Produced by FastQC (version 0.11.5)