FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623227
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53620.33032964582279617No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46290.2851726837959201No Hit
GAGTTACACCAACTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTT45700.28153794878966404No Hit
GTAATACAGGGCCGTGTCCTCAACTCTCAGACTGTTCATTTGTAGGTACA41440.25529392993093386No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40540.24974941890444158No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTC38950.23995411609097186No Hit
GTATTACTGTGCAAAAGATAGGTGGGCTGTAGCAGCAGTTGGCACGACAG36330.22381342843607208No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34620.21327885748573674No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33510.20644062721972958No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTTAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT31370.19325701211229238No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30710.18919103735953136No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27320.16830671249307708No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26930.16590409104826373No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26520.16337825824730615No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26200.16140687654899777No Hit
CAGTAATACAGGGCCGTGTCCTCAACTCTCAGACTGTTCATTTGTAGGTA26150.1610988481586371No Hit
GATCTATGCTGCATCCAATTTACACCCTGGGGTCCCATCAAGATTCAGCGGCACTGCTTCTGGGACAGATTTCA25710.15838819832346307No Hit
CTCCTGATCTATGCTGCATCCAATTTACACCCTGGGGTCCCATCAAGATT25600.15771053586466957No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24850.15309011000925934No Hit
CTGTATTACTGTGCAAAAGATAGGTGGGCTGTAGCAGCAGTTGGCACGAC24780.15265887026275438No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23990.1477920216950556No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23790.14655990813361286No Hit
GATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACCAA23780.1464983024555407No Hit
CTGTTGATGGTGAGAGTGAAATCTGTCCCAGAAGCAGTGCCGCTGAATCT23180.14280196177121254No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCC23090.1422475106685633No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC22560.13898240973074005No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22180.13664139396399888No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT22180.13664139396399888No Hit
CCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG22020.13565570311484468No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21700.13368432141653633No Hit
ACTTACTACTGTCAACAGAGTTACACCAACTGGACGTTCGGCCAAGGGAC21200.13060403751292948No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20990.12931031827341463No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20260.12481310377414866No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCAT20220.12456668106186011No Hit
GTCTGAGAGTTGAGGACACGGCCCTGTATTACTGTGCAAAAGATAGGTGG20220.12456668106186011No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20200.12444346970571583No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGAAGCAGTGCCG19730.12154800283632541No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19540.12037749495295483No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19470.11994625520644986No Hit
GAGTGAAATCTGTCCCAGAAGCAGTGCCGCTGAATCTTGATGGGACCCCA19200.11828290189850217No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATATCTGTTA19100.1176668451177808No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGAAGCAGTGCCGCTGAATCTTG19080.11754363376163654No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT18640.11483298392646253No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17880.11015095239298016No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17770.10947328993418666No Hit
GTGTAAATTGGATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAT17750.10935007857804238No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17750.10935007857804238No Hit
CACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACCAACTGGACGTTCGG17500.10780993662623897No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17470.10762511959202256No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17420.10731709120166187No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16610.10232703127781881No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16500.10164936881902531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4150.037.8112751
GTATCAA37200.036.6799431
ATCAACG42150.032.2164273
TCAACGC42550.032.1610644
AACGCAG43450.031.5724666
CAACGCA43500.031.3792785
TATCAAC43800.030.9980162
GCGTTAG450.0044309430.93411347
TCTGGAG23100.030.3952470
ACGCAGA46100.029.8315857
CGCAGAG46400.029.78588
GACAGGT3500.029.2391551
AGAGTAC47750.028.86243411
TAGAGCG605.781298E-428.437475
GCAGAGT48850.028.2919379
CTAATGG1506.691908E-527.26718770
CCGAAAC1209.299765E-725.71760668
GTATTAT801.0327707E-425.5842571
CCTATAC1501.1878001E-925.0203463
CAGAGTA56400.024.56437910
Produced by FastQC (version 0.11.5)