FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481992
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79020.5332012588462016No Hit
CTATTATGCTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT71740.484078186656878No Hit
GTTTTATACAGCTCCAGCAATAGGAATTACTTAGCTTGGTACCAGCAGAA65800.44399699863427067No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58140.3923098100394604No Hit
GCATAATAGTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC52780.3561422733725958No Hit
GAGTAGCATAATAGTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT46010.3104605153064254No Hit
ATGCTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA38780.26167482685466587No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37930.2559393033160773No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36980.24952901230236058No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35920.24237647706600307No Hit
GTATAAAACACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAAAGAGTCTGGAGA35780.24143180260082375No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35720.2410269421157469No Hit
GTTTATTACTGTCAACACTATTATGCTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA32890.22193102256962252No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA32600.21997419689175113No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31650.21356390587803442No Hit
TCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATTCCTATTGCTGGAGCTGTATAAAACACT30270.20425211472126706No Hit
CTCTTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATACAGCTCCAG27400.1848862881850914No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27090.1827945090121944No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27000.18218721828457915No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26280.17732889246365702No Hit
ACCCGGGACTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC25370.1711885084399916No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24850.1676797175693256No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24320.16410344995114684No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24050.16228157776830104No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24010.16201167077824982No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC23990.1618767172832242No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23670.15971746136281437No Hit
CTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGACTCCGGGGTCCCT23640.15951503112027596No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22860.154251844814277No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22350.15081053069112385No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21870.14757164681050908No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21820.14723426307294507No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21780.14696435608289382No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACACTATTATGCTACT21400.14440023967740717No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21150.142713320989587No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20490.13825985565374171No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20120.13576321599576785No Hit
AACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGAC19950.13461611128805015No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19730.1331316228427684No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG19590.1321869483775891No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19480.1314447041549482No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18910.12759852954671821No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGAGTCCCGGGT18610.12557422712133398No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18430.12435964566610345No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18330.1236848781909754No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18100.12213291299818083No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATTCCTATTGCTGGAGCTG18010.12152562227056557No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17810.12017608732030943No Hit
GTCCCGGGTAGATGCCCAGTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATT17800.12010861057279662No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17750.11977122683523257No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC17630.11896150586507889No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17630.11896150586507889No Hit
GTCAGGGACCCCGGAGTCCCGGGTAGATGCCCAGTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA17190.11599252897451538No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17130.11558766848943855No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16970.11450804052923363No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16280.10985214495084993No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15970.10776036577795291No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15780.1064783075752096No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15760.10634335408018396No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCTTTGGCTGTGTCTCTGGGCGAGAGGG15730.10614092383764555No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACACTATTATGCTACTCCGT15470.10438652840231256No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15270.10303699345205641No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15160.10229474922941555No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15100.10188988874433871No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14950.10087773753164661No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATACAGCTCCAGCAATAGGAATTA14920.10067530728910819No Hit
GTCTCCAGACTCTTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATA14840.10013549330900572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA100.0064248154149.8597670
CTAACGC453.8204234E-852.10993
CCTAACG453.8255166E-852.101112
GTGGTAT4550.041.2256361
GGTATCA13000.037.6183931
TGGTATC5050.037.1413842
GACAGGT2900.034.650861
GTATCAA28250.033.6736871
TGCGCTA950.00402212231.54942370
ATGCTCA31500.030.92344370
GCGGTAT500.004876307730.33391466
AATGGTA4700.030.29080270
ATCAACG32800.028.9032923
TATCAAC33000.028.6217782
CAACGCA33250.028.4094545
TCAACGC33300.028.3658394
AACGCAG33700.028.3282936
CGCAGAG34850.027.296468
ACGCAGA35200.027.0259577
AGAGTAC35550.026.9437811
Produced by FastQC (version 0.11.5)