FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530406
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA81650.5335185565137618No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC74830.48895521841916456No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTGCTGTGCCAAATCATACAATAATAGTCAG68610.4483124086026845No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG60890.39786827809091185No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG53900.3521941236508482No Hit
CACCTGCACCTTACGCAGTGGCATCAATGTTGGTACCTACAGGATATACTGGTATCAGCAGAGGCCAGGGAGTC53730.3510833073053817No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC51380.3357279048827566No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG51060.33363695646776087No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49040.3204378445981001No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGG47990.31357692011139526No Hit
CTCCAGAGCCCTGCTGCTTATCTGAGTCTGATTTGTACCTCAGGAGATATTGGGGAGGACTCCCTGGCCTCTG45970.30037780824173455No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC44330.28966169761488125No Hit
GGATATACTGGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATCTCCTG41980.2743062951922562No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA41840.2733915052606955No Hit
CCCCAATATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTC41020.2680334499472689No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG40770.26639989649805346No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC40720.2660731858082104No Hit
GGCCAGGGAGTCCTCCCCAATATCTCCTGAGGTACAAATCAGACTCAGAT40440.2642436059450891No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40210.26274073677181087No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT39090.25542241731932575No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC38270.2500643620058991No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG37830.24718930793527993No Hit
TCCTCAGACTGGAGCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGC37770.24679725510746822No Hit
CAATAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAAT37220.24320343751919427No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG36800.2404590677245123No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC36730.240001672758732No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT36680.23967496206888894No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35920.23470895958327395No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA35760.23366348537577608No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT35740.23353280109983887No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTGCTGTGCCAAATCATACAATAATAGTCAGC35540.2322259583404665No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA35520.2320952740645293No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA34860.2277826929586005No Hit
TCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTATTGTATGATTTGG33660.21994163640236641No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG32020.20922552577551318No Hit
ATGTTGGTACCTACAGGATATACTGGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATCTCCTGAGGTACAAAT31650.20680786667067433No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30590.1998816000460009No Hit
ATAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATCC30420.19877078370053436No Hit
CAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA30210.1973985988031934No Hit
ATCCTGTAGGTACCAACATTGATGCCACTGCGTAAGGTGCAGGTGAGACT29950.19569970321600935No Hit
CTATTATTGTATGATTTGGCACAGCAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC29530.19295533342132742No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA29280.19132177997211197No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC29160.19053767431648855No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28920.18896946300524176No Hit
ATATTGGGGAGGACTCCCTGGCCTCTGCTGATACCAGTATATCCTGTAGG28870.18864275231539868No Hit
GTCTGAGGATGAGGCTGACTATTATTGTATGATTTGGCACAGCAGCGCTTGGGTGTTCGGCGGAGGGACCAAG28360.18531030327899917No Hit
CCCAAGCGCTGCTGTGCCAAATCATACAATAATAGTCAGCCTCATCCTCA28160.18400346051962682No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT27930.18250059134634863No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG27890.18223922279447413No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27560.18008293224150979No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC27090.17701185175698475No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26770.174920903341989No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26520.17328734989277356No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT25870.1690401109248134No Hit
CTCCCTGGCCTCTGCTGATACCAGTATATCCTGTAGGTACCAACATTGATGCCACTGCGTAAGGTGCAGGTGAGA25400.16596903044028838No Hit
CTACAGGATATACTGGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATCTCCTGAGGTACAAATCAGACTCAGA25080.16387808202529264No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG25060.1637473977493554No Hit
GACTATTATTGTATGATTTGGCACAGCAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG24920.16283260781779477No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC24770.1618524757482655No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC24530.16028426443701868No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG24380.15930413236748941No Hit
GTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC24020.1569518154006192No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT23940.15642907829687022No Hit
GAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA23830.15571031477921546No Hit
CTACAGGATATACTGGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATC23740.15512223553749788No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23590.15414210346796864No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTG23450.15322731353640798No Hit
GATTTGTACCTCAGGAGATATTGGGGAGGACTCCCTGGCCTCTGCTGATA23240.15185512863906703No Hit
ATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGAC22500.14701981042938933No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA22420.14649707332564038No Hit
GTCTCACCTGCACCTTACGCAGTGGCATCAATGTTGGTACCTACAGGATA22270.14551694125611112No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22230.14525557270423664No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC22160.14479817773845632No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT21990.14368736139298985No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGCGCTGCTGTGCCAAATCATACAATAATAGTCAGCCTCATCCTCAGA21490.14042025449455894No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG20960.13695712118222222No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT20550.1342780935255089No Hit
GCTTATCTGAGTCTGATTTGTACCTCAGGAGATATTGGGGAGGACTCCCTGGCCTCTGCTGATACCAGTATATC20200.13199111869660732No Hit
GCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAG20170.13179509228270145No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20140.13159906586879558No Hit
GCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGC20020.13081496021317218No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC20000.13068427593723497No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19840.12963880172973707No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19100.12480348352005939No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19070.12460745710615354No Hit
GGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCA18820.12297390365693808No Hit
TCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGCCCTGCTGCTTATC18730.12238582441522054No Hit
GATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTATT18710.12225514013928329No Hit
CTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCT18580.12140569234569128No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC18520.12101363951787956No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18510.12094829737991095No Hit
CTGCATCTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGGC18010.11768119048148008No Hit
GTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTG17670.11545955779054709No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17620.11513284710070398No Hit
CCTTACGCAGTGGCATCAATGTTGGTACCTACAGGATATACTGGTATCAG17490.11428339930711197No Hit
GTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGG17410.11376066220336303No Hit
GGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCT17350.11336860937555132No Hit
GTATATCCTGTAGGTACCAACATTGATGCCACTGCGTAAGGTGCAGGTGA17330.1132379250996141No Hit
GCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC17320.11317258296164547No Hit
ATACTGGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATCTCCTGAGGT17280.11291121440977099No Hit
CTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT17220.1125191615819593No Hit
GTGCCAAATCATACAATAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATCCCTGCATTGGC17140.11199642447821037No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC17040.11134300309852418No Hit
GGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTT17000.11108163454664971No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16820.10990547606321459No Hit
AGTATATCCTGTAGGTACCAACATTGATGCCACTGCGTAAGGTGCAGGTGAGACTGGCTGATGCTCCAGGAGATG16820.10990547606321459No Hit
GGATGAGGCTGACTATTATTGTATGATTTGGCACAGCAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGT16710.1091867125455598No Hit
CTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCC16670.10892534399368534No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT16600.108467949027905No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16410.10722644840650128No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG16180.10572357923322308No Hit
CTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGGCATCAAT15930.10409002578400765No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC15920.10402468364603902No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG15790.103175235852447No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG15790.103175235852447No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG15630.10212976164494914No Hit
GAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTC15520.10141099812729433No Hit
GATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTA15480.10114962957541987No Hit
ATATACTGGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATCTCCTGAGGTACAAATCAGACTCAGATAAGCAG15410.10069223460963954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT406.0746468E-573.8628470
AGGGGGG3600.069.7593470
GCGTATG252.591126E-455.09749240
GTGGTAT2250.045.3758051
CGTATGC3600.042.151141
AGACGTA4650.041.71237625
GTACGTA653.8536527E-641.3830769
GACGTAG4650.040.99129526
CGTAGAG4750.040.16114428
TCGTATG3450.039.92572440
ACGTAGA4800.039.724627
ACGAGAC5000.039.4627622
CTTATAC5100.039.3701821
TATACAC5200.039.249563
TTATACA5150.038.9701162
GTATGCC3900.038.96937642
CACGAGA5150.037.65155421
TCTCGTA3050.037.149238
ATGCCGT4200.037.1129144
GAGACGT5250.036.9392124
Produced by FastQC (version 0.11.5)